Enhancement of Iron Acquisition in Rice by the Mugineic Acid Synthase Gene With Ferric Iron Reductase Gene and Confers Tolerance in Submerged and Nonsubmerged Calcareous Soils

Overview

Journal Front Plant Sci

Date 2019 Nov 5

PMID 31681346

Citations 10

Authors

Hiroshi Masuda

May Sann Aung

Takanori Kobayashi

Tatsuro Hamada

Naoko K Nishizawa

Affiliations

Soon will be listed here.

Abstract

Iron (Fe) is an essential micronutrient for plants. Plants encounter Fe deficiency when grown in calcareous soil with low Fe availability, leading to reduced crop yield and agricultural problem. Rice acquires Fe from the soil Strategy I-related system (ferrous ion uptake by OsIRT1) and Strategy II system (ferric ion uptake by chelation). However, rice plants have a weak ability in Fe(III) reduction and phytosiderophore secretion. We previously produced an Fe deficiency-tolerant rice harboring promoter- (for higher Fe(III) reductase ability) and a promoter- (for higher phytosiderophore secretion). In this study, we produced a new Fe deficiency-tolerant rice by the additional introduction of a barley genome fragment with and (named as IRI lines) for further enhancement in Strategy II phytosiderophore productivity and better growth performance in various environments. Our results show that an enhanced tolerance was observed in introduced line at the early growth stage, introduced line in the late stage, and RI line in all stages among five types of cultivation method. Moreover, we demonstrated that new IRI rice lines exhibited enhanced tolerance to Fe deficiency compared to nontransgenic (NT) rice and rice lines harboring the overexpressing or the fragment under submerged calcareous soil. The yields of IRI lines were ninefold higher than the NT line. Furthermore, under Fe-limited nonsubmerged calcareous soil condition (a new cultivation condition), IRI lines also conferred enhanced tolerance than NT, lines introducing only the promoter- or overexpressing , and lines harboring both. Our results demonstrate that further enhancement of the Strategy II Fe uptake system by the mugineic acid synthase gene in addition to Fe uptake by enhanced ferric Fe reduction and phytosiderophore production in rice contributes Fe deficiency tolerance and broaden its utility in calcareous soil cultivation under paddy or nonpaddy field conditions.

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