The Immunological Properties of Recombinant Multi-Cystatin-Like Domain Protein From Produced in Yeast

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2019 Nov 5

PMID 31681308

Citations 5

Authors

Anna Stachyra

Anna Zawistowska-Deniziak

Katarzyna Basalaj

Sylwia Grzelak

Michal Gondek

Justyna Bien-Kalinowska

Affiliations

Soon will be listed here.

Abstract

Trichinellosis is a globally-distributed zoonotic parasitic disease caused by nematode worms of the genus . One of the most common species of known to affect human health is ; however, it is relatively poorly investigated. A thorough knowledge of the proteins expressed by is important when developing immunological detection methods and vaccines and studying its interactions with the host. The present study uses the expression system to produce a soluble TbCLP antigen which induces strong antibody responses in the host during natural infection. Our results demonstrate the feasibility of TbCLP antigen production in yeasts, which are able to carry out post-translational modifications such as glycosylation and disulfide bond formation; they also indicate that the glycosylated TbCLP antigen had immunogenic effects in the tested mice and induced a mixed Th1/Th2 response, and was associated with a reduced larval burden after challenge with . Subsequent stimulation of mice splenocytes revealed that TbCLP most likely possesses immunomodulatory properties and may play a significant role in the early phase of infection, affecting host immunological responses.

Citing Articles

cystatin has protective effects against "two-hit" sepsis in mice by regulating the inflammatory microenvironment.

Duo W, Wang Y, Wang J, Xu X, Li L, Yang D Nan Fang Yi Ke Da Xue Xue Bao. 2025; 45(1):110-117.

PMID: 39819719 PMC: 11744279. DOI: 10.12122/j.issn.1673-4254.2025.01.14.

Secreted and surface proteome and transcriptome of .

Wong Y, Pearson M, Fedorova O, Ivanov V, Khmelevskaya E, Tedla B Front Parasitol. 2025; 2():1195457.

PMID: 39816815 PMC: 11732047. DOI: 10.3389/fpara.2023.1195457.

Th1 and Th2 epitopes of Cowdria polymorphic gene 1 of Ehrlichia ruminantium.

Ngoepe T, Pretorius A, Steyn H, Van Kleef M Onderstepoort J Vet Res. 2023; 90(1):e1-e15.

PMID: 37042556 PMC: 10091069. DOI: 10.4102/ojvr.v90i1.2070.

Immunoproteomic Analysis of Proteins Recognized by IgG Antibodies from Meat Juice of Carnivores Naturally Infected with .

Cybulska A Pathogens. 2022; 11(10).

PMID: 36297212 PMC: 9612144. DOI: 10.3390/pathogens11101155.

Proteomics of Gnathostomiasis: A Way Forward for Diagnosis and Treatment Development.

Thiangtrongjit T, Nogrado K, Ketboonlue T, Malaitong P, Adisakwattana P, Reamtong O Pathogens. 2021; 10(9).

PMID: 34578113 PMC: 8465481. DOI: 10.3390/pathogens10091080.

References

Liu R, Wang Z, Wang L, Long S, Ren H, Cui J . Analysis of differentially expressed genes of Trichinella spiralis larvae activated by bile and cultured with intestinal epithelial cells using real-time PCR. Parasitol Res. 2013; 112(12):4113-20. DOI: 10.1007/s00436-013-3602-1. View

Wei J, Versteeg L, Liu Z, Keegan B, Gazzinelli-Guimaraes A, Fujiwara R . Yeast-expressed recombinant As16 protects mice against Ascaris suum infection through induction of a Th2-skewed immune response. PLoS Negl Trop Dis. 2017; 11(7):e0005769. PMC: 5529013. DOI: 10.1371/journal.pntd.0005769. View

Gonzalez-Hernandez A, Borloo J, Peelaers I, Casaert S, Leclercq G, Claerebout E . Comparative analysis of the immune responses induced by native versus recombinant versions of the ASP-based vaccine against the bovine intestinal parasite Cooperia oncophora. Int J Parasitol. 2017; 48(1):41-49. DOI: 10.1016/j.ijpara.2017.07.002. View

Hartmann S, Lucius R . Modulation of host immune responses by nematode cystatins. Int J Parasitol. 2003; 33(11):1291-302. DOI: 10.1016/s0020-7519(03)00163-2. View

Turk V, Bode W . The cystatins: protein inhibitors of cysteine proteinases. FEBS Lett. 1991; 285(2):213-9. DOI: 10.1016/0014-5793(91)80804-c. View

Wu X, Fu B, Wang X, Yu L, Yu S, Deng H . Identification of antigenic genes in Trichinella spiralis by immunoscreening of cDNA libraries. Vet Parasitol. 2008; 159(3-4):272-5. DOI: 10.1016/j.vetpar.2008.10.035. View

Martinon S, Cisneros A, Villicana S, Hernandez-Miramontes R, Mixcoha E, Calderon-Vargas P . Chemical and Immunological Characteristics of Aluminum-Based, Oil-Water Emulsion, and Bacterial-Origin Adjuvants. J Immunol Res. 2019; 2019:3974127. PMC: 6530223. DOI: 10.1155/2019/3974127. View

Dupouy-Camet J . Trichinellosis: a worldwide zoonosis. Vet Parasitol. 2000; 93(3-4):191-200. DOI: 10.1016/s0304-4017(00)00341-1. View

Malek T, Castro I . Interleukin-2 receptor signaling: at the interface between tolerance and immunity. Immunity. 2010; 33(2):153-65. PMC: 2946796. DOI: 10.1016/j.immuni.2010.08.004. View

10.

Pozio E, Zarlenga D . New pieces of the Trichinella puzzle. Int J Parasitol. 2013; 43(12-13):983-97. DOI: 10.1016/j.ijpara.2013.05.010. View

11.

Sun G, Song Y, Jiang P, Na Ren H, Yan S, Han Y . Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool. PLoS Negl Trop Dis. 2018; 12(5):e0006485. PMC: 5967804. DOI: 10.1371/journal.pntd.0006485. View

12.

Cybulska A, Kornacka A, Moskwa B . The occurrence and muscle distribution of in raccoon dogs () in wildlife in the Głęboki Bród Forest District, Poland. Int J Parasitol Parasites Wildl. 2019; 9:149-153. PMC: 6523818. DOI: 10.1016/j.ijppaw.2019.05.003. View

13.

Holzhausen J, Haake C, Schicht S, Hinse P, Jordan D, Kremmer E . Biological function of Dictyocaulus viviparus asparaginyl peptidase legumain-1 and its suitability as a vaccine target. Parasitology. 2017; 145(3):378-392. DOI: 10.1017/S0031182017001573. View

14.

Yang Z, Li W, Yang Z, Pan A, Liao W, Zhou X . A novel antigenic cathepsin B protease induces protective immunity in Trichinella-infected mice. Vaccine. 2017; 36(2):248-255. DOI: 10.1016/j.vaccine.2017.11.048. View

15.

Cooper D, Eleftherianos I . Parasitic Nematode Immunomodulatory Strategies: Recent Advances and Perspectives. Pathogens. 2016; 5(3). PMC: 5039438. DOI: 10.3390/pathogens5030058. View

16.

Ilic N, Gruden-Movsesijan A, Sofronic-Milosavljevic L . Trichinella spiralis: shaping the immune response. Immunol Res. 2012; 52(1-2):111-9. DOI: 10.1007/s12026-012-8287-5. View

17.

Bilska-Zajac E, Rozycki M, Chmurzynska E, Marucci G, Cencek T, Karamon J . Trichinella species circulating in wild boar (Sus scrofa) populations in Poland. Int J Parasitol Parasites Wildl. 2014; 2:211-3. PMC: 3862528. DOI: 10.1016/j.ijppaw.2013.05.004. View

18.

Couper K, Blount D, Riley E . IL-10: the master regulator of immunity to infection. J Immunol. 2008; 180(9):5771-7. DOI: 10.4049/jimmunol.180.9.5771. View

19.

Ochieng J, Chaudhuri G . Cystatin superfamily. J Health Care Poor Underserved. 2010; 21(1 Suppl):51-70. PMC: 2888135. DOI: 10.1353/hpu.0.0257. View

20.

Xu N, Liu X, Tang B, Wang L, Shi H, Boireau P . Recombinant Serine Protease Inhibitors Alter Macrophage Polarization . Front Microbiol. 2017; 8:1834. PMC: 5613137. DOI: 10.3389/fmicb.2017.01834. View