Molecular Characterization of Aquaglyceroporine: A Novel Mutation in from (MRHO/IR/75/ER)

Overview

Journal Iran J Parasitol

Publisher Tehran University of Medical Sciences

Specialty Parasitology

Date 2019 Nov 2

PMID 31673266

Citations 1

Authors

Gilda Eslami

Maryam Ghavami

Ali Reza Moradi

Hamid Nadri

Salman Ahmadian

Affiliations

Soon will be listed here.

Abstract

Background: The first line treatment for cutaneous leishmaniasis is pentavalent antimony such as sodium stibogluconate (pentostam) and meglumine antimonite (glucantime). One of the most important ways to uptake the drug is by a trans-membrane protein, called aquaglyceroporin encoded by (). In this study, molecular characterization of was reported.

Methods: (MRHO/IR/75/ER) promastigotes were cultured, and then DNA extraction and RNA extraction were done and followed by cDNA synthesis. Amplicons resulted from PCR and RT-PCR using specific primers were purified and sequenced. Molecular characterization was done by bioinformatically software such as BLST, ClustalW2, and RMSD.

Results: Amplicons resulted from PCR and RT-PCR showed equal size in length. BLASTn analysis showed a point nucleotide change in gene that encoded 282-amino-acid long protein with a mutation at position 154 including replacement of alanine by threonine. The observed mutation in the interested gene was assessed using the above-mentioned software. The mentioned gene was submitted at GenBank, NCBI with accession number of KU514052.

Conclusion: The functional prediction of the protein encoded from showed that the mentioned mutation could not affect the three-dimension structure, but it may modify the drug uptake potential of this important channel. Based on from role, it seems to be an appropriate candidate for drug development. According to search through internet, this is the first report of from (MRHO/IR/75/ER).

Citing Articles

Molecular characteristic of treatment failure clinical isolates of .

Eslami G, Hatefi S, Ramezani V, Tohidfar M, Churkina T, Orlov Y PeerJ. 2021; 9:e10969.

PMID: 33763300 PMC: 7956003. DOI: 10.7717/peerj.10969.

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