Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Sp. BY01

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2019 Nov 2

PMID 31671673

Citations 12

Authors

Yue Yang

Zhou Zheng

Yifei Xiao

Jiaojiao Zhang

Yu Zhou

Xiao Li

Shangyong Li

Huiqing Yu

Affiliations

Soon will be listed here.

Abstract

Chitosanase plays an important role in the production of chitooligosaccharides (CHOS), which possess various biological activities. Herein, a glycoside hydrolase (GH) family 46 chitosanase-encoding gene, , was cloned from marine bacterium sp. BY01 and heterologously expressed in . The recombinant chitosanase, CsnB, was optimally active at 35 °C and pH 5.0. It was also revealed to be a cold-adapted enzyme, maintaining 39.5% and 40.4% of its maximum activity at 0 and 10 °C, respectively. Meanwhile, CsnB showed wide pH-stability within the range of pH 3.0 to 7.0. Then, an improved reaction condition was built to enhance its thermostability with a final glycerol volume concentration of 20%. Moreover, CsnB was determined to be an endo-type chitosanase, yielding chitosan disaccharides and trisaccharides as the main products. Overall, CsnB provides a new choice for enzymatic CHOS production.

Citing Articles

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References

Jayakumar R, Prabaharan M, Nair S, Tamura H . Novel chitin and chitosan nanofibers in biomedical applications. Biotechnol Adv. 2009; 28(1):142-50. DOI: 10.1016/j.biotechadv.2009.11.001. View

Senel S, McClure S . Potential applications of chitosan in veterinary medicine. Adv Drug Deliv Rev. 2004; 56(10):1467-80. DOI: 10.1016/j.addr.2004.02.007. View

Chen X, Bhandari B, Zhou P . Insight into the effect of glycerol on stability of globular proteins in high protein model system. Food Chem. 2018; 278:780-785. DOI: 10.1016/j.foodchem.2018.11.117. View

Roy S, Fortin M, Gagnon J, Ghinet M, LeHoux J, Dupuis G . Quantitative fluorometric analysis of the protective effect of chitosan on thermal unfolding of catalytically active native and genetically-engineered chitosanases. Biochim Biophys Acta. 2007; 1774(8):975-84. DOI: 10.1016/j.bbapap.2007.05.016. View

Cheng C, Chang C, Wu Y, Li Y . Exploration of glycosyl hydrolase family 75, a chitosanase from Aspergillus fumigatus. J Biol Chem. 2005; 281(6):3137-44. DOI: 10.1074/jbc.M512506200. View

Weikert T, Niehues A, Cord-Landwehr S, Hellmann M, Moerschbacher B . Reassessment of chitosanase substrate specificities and classification. Nat Commun. 2017; 8(1):1698. PMC: 5700058. DOI: 10.1038/s41467-017-01667-1. View

Zhou Y, Chen X, Li X, Han Y, Wang Y, Yao R . Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium sp. SY39. Molecules. 2019; 24(1). PMC: 6337222. DOI: 10.3390/molecules24010183. View

Qin Z, Chen Q, Lin S, Luo S, Qiu Y, Zhao L . Expression and characterization of a novel cold-adapted chitosanase suitable for chitooligosaccharides controllable preparation. Food Chem. 2018; 253:139-147. DOI: 10.1016/j.foodchem.2018.01.137. View

Hao C, Wang W, Wang S, Zhang L, Guo Y . An Overview of the Protective Effects of Chitosan and Acetylated Chitosan Oligosaccharides against Neuronal Disorders. Mar Drugs. 2017; 15(4). PMC: 5408235. DOI: 10.3390/md15040089. View

10.

Gerday C, Aittaleb M, Bentahir M, Chessa J, Claverie P, Collins T . Cold-adapted enzymes: from fundamentals to biotechnology. Trends Biotechnol. 2000; 18(3):103-7. DOI: 10.1016/s0167-7799(99)01413-4. View

11.

Johnsen M, Hansen O, Stougaard P . Isolation, characterization and heterologous expression of a novel chitosanase from Janthinobacterium sp. strain 4239. Microb Cell Fact. 2010; 9:5. PMC: 2835661. DOI: 10.1186/1475-2859-9-5. View

12.

Yu S, Kwon Y, Lee C, Apostolidis E, Kim Y . Antidiabetic effect of chitosan oligosaccharide (GO2KA1) is mediated via inhibition of intestinal alpha-glucosidase and glucose transporters and PPARγ expression. Biofactors. 2016; 43(1):90-99. DOI: 10.1002/biof.1311. View

13.

Wan A, Tai B . CHITIN--a promising biomaterial for tissue engineering and stem cell technologies. Biotechnol Adv. 2013; 31(8):1776-85. DOI: 10.1016/j.biotechadv.2013.09.007. View

14.

Cantarel B, Coutinho P, Rancurel C, Bernard T, Lombard V, Henrissat B . The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics. Nucleic Acids Res. 2008; 37(Database issue):D233-8. PMC: 2686590. DOI: 10.1093/nar/gkn663. View

15.

Pan H, Fu C, Huang L, Jiang Y, Deng X, Guo J . Anti-Obesity Effect of Chitosan Oligosaccharide Capsules (COSCs) in Obese Rats by Ameliorating Leptin Resistance and Adipogenesis. Mar Drugs. 2018; 16(6). PMC: 6025060. DOI: 10.3390/md16060198. View

16.

Choi Y, Kim E, Piao Z, Yun Y, Shin Y . Purification and characterization of chitosanase from Bacillus sp. strain KCTC 0377BP and its application for the production of chitosan oligosaccharides. Appl Environ Microbiol. 2004; 70(8):4522-31. PMC: 492396. DOI: 10.1128/AEM.70.8.4522-4531.2004. View

17.

Muanprasat C, Chatsudthipong V . Chitosan oligosaccharide: Biological activities and potential therapeutic applications. Pharmacol Ther. 2016; 170:80-97. DOI: 10.1016/j.pharmthera.2016.10.013. View

18.

Tharanathan R, Kittur F . Chitin--the undisputed biomolecule of great potential. Crit Rev Food Sci Nutr. 2003; 43(1):61-87. DOI: 10.1080/10408690390826455. View

19.

Zhang J, Cao H, Li S, Zhao Y, Wang W, Xu Q . Characterization of a new family 75 chitosanase from Aspergillus sp. W-2. Int J Biol Macromol. 2015; 81:362-9. DOI: 10.1016/j.ijbiomac.2015.08.026. View

20.

Liu X, Zhou P, Tran A, Labuza T . Effects of polyols on the stability of whey proteins in intermediate-moisture food model systems. J Agric Food Chem. 2009; 57(6):2339-45. DOI: 10.1021/jf802789y. View