Comparative Analysis of Aerotolerance, Antibiotic Resistance, and Virulence Gene Prevalence in Isolates from Retail Raw Chicken and Duck Meat in South Korea

Overview

Journal Microorganisms

Specialty Microbiology

Date 2019 Oct 30

PMID 31658662

Citations 22

Authors

JinShil Kim

Hyeeun Park

Junhyung Kim

Jong Hyun Kim

Jae In Jung

Seongbeom Cho

Sangryeol Ryu

Byeonghwa Jeon

Affiliations

Soon will be listed here.

Abstract

Human infections with are primarily associated with the consumption of contaminated poultry meat. In this study, we isolated from retail raw chicken and duck meat in Korea and compared their aerotolerance, antibiotic resistance, and virulence gene prevalence. Whereas isolates from chicken dominantly belonged to multilocus sequence typing (MLST) clonal complex (CC)-21, CC-45 is the common MLST sequence type in duck meat isolates. strains from both chicken and duck meat were highly tolerant to aerobic stress. The prevalence of virulence genes was higher in strains from chicken than those from duck meat. However, antibiotic resistance was higher in duck meat isolates than chicken isolates. Based on the prevalence of virulence genes and antibiotic resistance, fluoroquinolone-resistant strains harboring all tested virulence genes except B11 were predominant on retail poultry. Fluoroquinolone-resistant strains carrying most virulence genes were more frequently isolated in summer than in winter. The comparative profiling analysis in this study successfully demonstrated that antibiotic-resistant and pathogenic strains of are highly prevalent on retail poultry and that retail duck meat is an important vehicle potentially transmitting to humans in Korea.

Citing Articles

Comprehensive analysis of antimicrobial resistance dynamics among broiler and duck intensive production systems.

Szoke Z, Fauszt P, Mikolas M, David P, Szilagyi-Tolnai E, Pesti-Asboth G Sci Rep. 2025; 15(1):4673.

PMID: 39920217 PMC: 11806100. DOI: 10.1038/s41598-025-89432-z.

Aerotolerancy of spp.: A Comprehensive Review.

Delaporte E, Karki A, Fakhr M Pathogens. 2024; 13(10).

PMID: 39452714 PMC: 11510350. DOI: 10.3390/pathogens13100842.

A Decade of Antimicrobial Resistance in Human and Animal spp. Isolates.

Barata R, Saavedra M, Almeida G Antibiotics (Basel). 2024; 13(9).

PMID: 39335077 PMC: 11429304. DOI: 10.3390/antibiotics13090904.

Pathogenic Bacteria in Free-Living Birds, and Its Public Health Significance.

Kobuszewska A, Wysok B Animals (Basel). 2024; 14(6).

PMID: 38540066 PMC: 10967383. DOI: 10.3390/ani14060968.

Effect of biofilm formation in a hostile oxidative stress environment on the survival of recovered from poultry in Iraqi markets.

Kanaan M Vet World. 2024; 17(1):136-142.

PMID: 38406363 PMC: 10884572. DOI: 10.14202/vetworld.2024.136-142.

References

Lara-Tejero M, Galan J . A bacterial toxin that controls cell cycle progression as a deoxyribonuclease I-like protein. Science. 2000; 290(5490):354-7. DOI: 10.1126/science.290.5490.354. View

Bolton F, Coates D . A study of the oxygen and carbon dioxide requirements of thermophilic campylobacters. J Clin Pathol. 1983; 36(7):829-34. PMC: 498399. DOI: 10.1136/jcp.36.7.829. View

Rodrigues R, Pocheron A, Hernould M, Haddad N, Tresse O, Cappelier J . Description of Campylobacter jejuni Bf, an atypical aero-tolerant strain. Gut Pathog. 2015; 7:30. PMC: 4653858. DOI: 10.1186/s13099-015-0077-x. View

Adzitey F, Rusul G, Huda N, Cogan T, Corry J . Prevalence, antibiotic resistance and RAPD typing of Campylobacter species isolated from ducks, their rearing and processing environments in Penang, Malaysia. Int J Food Microbiol. 2012; 154(3):197-205. DOI: 10.1016/j.ijfoodmicro.2012.01.006. View

Luangtongkum T, Jeon B, Han J, Plummer P, Logue C, Zhang Q . Antibiotic resistance in Campylobacter: emergence, transmission and persistence. Future Microbiol. 2009; 4(2):189-200. PMC: 2691575. DOI: 10.2217/17460913.4.2.189. View

Aarestrup F, Engberg J . Antimicrobial resistance of thermophilic Campylobacter. Vet Res. 2001; 32(3-4):311-21. DOI: 10.1051/vetres:2001127. View

Yamazaki-Matsune W, Taguchi M, Seto K, Kawahara R, Kawatsu K, Kumeda Y . Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. J Med Microbiol. 2007; 56(Pt 11):1467-1473. DOI: 10.1099/jmm.0.47363-0. View

Allos B . Campylobacter jejuni Infections: update on emerging issues and trends. Clin Infect Dis. 2001; 32(8):1201-6. DOI: 10.1086/319760. View

Oh E, Andrews K, McMullen L, Jeon B . Tolerance to stress conditions associated with food safety in Campylobacter jejuni strains isolated from retail raw chicken. Sci Rep. 2019; 9(1):11915. PMC: 6695378. DOI: 10.1038/s41598-019-48373-0. View

10.

Kim N, Jung S, Na H, Chung G, Yoo C, Seong W . Enteric Bacteria Isolated from Diarrheal Patients in Korea in 2014. Osong Public Health Res Perspect. 2015; 6(4):233-40. PMC: 4588440. DOI: 10.1016/j.phrp.2015.07.005. View

11.

Engberg J, Aarestrup F, Taylor D, Gerner-Smidt P, Nachamkin I . Quinolone and macrolide resistance in Campylobacter jejuni and C. coli: resistance mechanisms and trends in human isolates. Emerg Infect Dis. 2001; 7(1):24-34. PMC: 2631682. DOI: 10.3201/eid0701.010104. View

12.

Han K, Jang S, Choo E, Heu S, Ryu S . Prevalence, genetic diversity, and antibiotic resistance patterns of Campylobacter jejuni from retail raw chickens in Korea. Int J Food Microbiol. 2007; 114(1):50-9. DOI: 10.1016/j.ijfoodmicro.2006.10.042. View

13.

Mohan V, Stevenson M, Marshall J, Fearnhead P, Holland B, Hotter G . Campylobacter jejuni colonization and population structure in urban populations of ducks and starlings in New Zealand. Microbiologyopen. 2013; 2(4):659-73. PMC: 3831628. DOI: 10.1002/mbo3.102. View

14.

Ziprin R, Young C, Stanker L, Hume M, Konkel M . The absence of cecal colonization of chicks by a mutant of Campylobacter jejuni not expressing bacterial fibronectin-binding protein. Avian Dis. 1999; 43(3):586-9. View

15.

Konkel M, Kim B, Garvis S . Bacterial secreted proteins are required for the internaliztion of Campylobacter jejuni into cultured mammalian cells. Mol Microbiol. 1999; 32(4):691-701. DOI: 10.1046/j.1365-2958.1999.01376.x. View

16.

Wei B, Cha S, Kang M, Roh J, Seo H, Yoon R . Antimicrobial susceptibility profiles and molecular typing of Campylobacter jejuni and Campylobacter coli isolates from ducks in South Korea. Appl Environ Microbiol. 2014; 80(24):7604-10. PMC: 4249223. DOI: 10.1128/AEM.02469-14. View

17.

Guerry P . Campylobacter flagella: not just for motility. Trends Microbiol. 2007; 15(10):456-61. DOI: 10.1016/j.tim.2007.09.006. View

18.

Konkel M, Garvis S, Tipton S, Anderson Jr D, Cieplak Jr W . Identification and molecular cloning of a gene encoding a fibronectin-binding protein (CadF) from Campylobacter jejuni. Mol Microbiol. 1997; 24(5):953-63. DOI: 10.1046/j.1365-2958.1997.4031771.x. View

19.

Hanning I, Biswas D, Herrera P, Roesler M, Ricke S . Prevalence and characterization of Campylobacter jejuni isolated from pasture flock poultry. J Food Sci. 2011; 75(7):M496-502. DOI: 10.1111/j.1750-3841.2010.01747.x. View

20.

Jorgensen F, Ellis-Iversen J, Rushton S, Bull S, Harris S, Bryan S . Influence of season and geography on Campylobacter jejuni and C. coli subtypes in housed broiler flocks reared in Great Britain. Appl Environ Microbiol. 2011; 77(11):3741-8. PMC: 3127617. DOI: 10.1128/AEM.02444-10. View