Molecular Identification of Non- Yeasts Associated with Pigeon Droppings in Shiraz, Southern Iran

Overview

Journal Iran J Vet Res

Specialty Veterinary Medicine

Date 2019 Oct 29

PMID 31656526

Citations 4

Authors

K Pakshir

Z Zareshahrabadi

K Zomorodian

S Ansari

H Nouraei

A Gharavi

Affiliations

Soon will be listed here.

Abstract

Background: Birds are considered as a reservoir for pathogenic and non-pathogenic fungi. Pigeon droppings have the potential for spreading these fungi to the environment. species are important fungi associated with pigeon droppings. In this regard, there are many types of yeast associated with guano that is important for human and animal health.

Aims: The main objective of this study is the identification of non- yeasts isolated from pigeon dropping in Shiraz, Southern Iran.

Methods: A total of 100 unknown yeasts, which were previously screened and identified as non- from pigeon guano through the conventional methods, were used in this study. Identification of the isolates was performed based on conventional methods and DNA sequence analysis of internal transcribed spacer (ITS) rDNA gene region. The sequence results were deposited in NCBI database using the Basic Local Alignment Search Tool (BLAST).

Results: A total of 16 species belonging to 7 genera were identified as spp. 51% (8 species), sp. 24%, spp. 21% (3 species), 2%, 1%, 1%, and 1%. The predominant isolates were (24%), (20%), and (13%). The other species were 2 (2%), 1 (1%), 1 (1%), and 1 (1%).

Conclusion: Pigeon excreta examined in this study were associated with several kinds of opportunistic yeasts which could cause diseases in prone human and animals.

Citing Articles

Molecular characterization of non- yeast communities isolated from trees.

Nouraei H, Gharechahi F, Zareshahrabadi Z, Zomorodian K, Gharavi A, Khodadadi H Curr Med Mycol. 2025; 10.

PMID: 39744339 PMC: 11686939. DOI: 10.22034/CMM.2024.345184.1500.

Identification of Pathogenic and Opportunistic Yeasts in Pigeon Excreta by MALDI-TOF Mass Spectrometry and Their Prevalence in Chon Buri Province, Thailand.

Nualmalang R, Thanomsridetchai N, Teethaisong Y, Sukphopetch P, Tangwattanachuleeporn M Int J Environ Res Public Health. 2023; 20(4).

PMID: 36833884 PMC: 9967633. DOI: 10.3390/ijerph20043191.

and Other Opportunistic Species in Pigeon Dropping in Saudi Arabia: Identification and Characterization by DNA Sequencing.

Sirag B, Khidir E, Dumyati M, Sindi B, Alsinnari M, Faidah H Front Microbiol. 2021; 12:726203.

PMID: 34707582 PMC: 8544600. DOI: 10.3389/fmicb.2021.726203.

Uncommon Non- Yeasts in Healthy Turkeys-Antimicrobial Susceptibility and Biochemical Characteristic of Trichosporon Isolates.

Bobrek K, Sokol I, Gawel A Pathogens. 2021; 10(5).

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References

Chen S, Marriott D, Playford E, Nguyen Q, Ellis D, Meyer W . Candidaemia with uncommon Candida species: predisposing factors, outcome, antifungal susceptibility, and implications for management. Clin Microbiol Infect. 2009; 15(7):662-9. DOI: 10.1111/j.1469-0691.2009.02821.x. View

Makimura K, Murayama S, Yamaguchi H . Detection of a wide range of medically important fungi by the polymerase chain reaction. J Med Microbiol. 1994; 40(5):358-64. DOI: 10.1099/00222615-40-5-358. View

Miceli M, Diaz J, Lee S . Emerging opportunistic yeast infections. Lancet Infect Dis. 2011; 11(2):142-51. DOI: 10.1016/S1473-3099(10)70218-8. View

Wirth F, Goldani L . Epidemiology of Rhodotorula: an emerging pathogen. Interdiscip Perspect Infect Dis. 2012; 2012:465717. PMC: 3469092. DOI: 10.1155/2012/465717. View

Bellemain E, Carlsen T, Brochmann C, Coissac E, Taberlet P, Kauserud H . ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases. BMC Microbiol. 2010; 10:189. PMC: 2909996. DOI: 10.1186/1471-2180-10-189. View

Asada N, Uryu H, Koseki M, Takeuchi M, Komatsu M, Matsue K . Successful treatment of breakthrough Trichosporon asahii fungemia with voriconazole in a patient with acute myeloid leukemia. Clin Infect Dis. 2006; 43(4):e39-41. DOI: 10.1086/505970. View

Mancianti F, Nardoni S, Ceccherelli R . Occurrence of yeasts in psittacines droppings from captive birds in Italy. Mycopathologia. 2002; 153(3):121-4. DOI: 10.1023/a:1014576304894. View

Rosario Medina I, Roman Fuentes L, Batista Arteaga M, Real Valcarcel F, Acosta Arbelo F, Padilla Del Castillo D . Pigeons and their droppings as reservoirs of Candida and other zoonotic yeasts. Rev Iberoam Micol. 2017; 34(4):211-214. DOI: 10.1016/j.riam.2017.03.001. View

Pakshir K, Fakhim H, Vaezi A, Meis J, Mahmoodi M, Zomorodian K . Molecular epidemiology of environmental Cryptococcus species isolates based on amplified fragment length polymorphism. J Mycol Med. 2018; 28(4):599-605. DOI: 10.1016/j.mycmed.2018.09.005. View

10.

Kaygusuz I, Mulazimoglu L, Cerikcioglu N, Toprak A, Oktay A, Korten V . An unusual native tricuspid valve endocarditis caused by Candida colliculosa. Clin Microbiol Infect. 2003; 9(4):319-22. DOI: 10.1046/j.1469-0691.2003.00511.x. View

11.

Bieganska M, Rzewuska M, Dabrowska I, Malewska-Biel B, Ostrzeszewicz M, Dworecka-Kaszak B . Mixed Infection of Respiratory Tract in a Dog Caused by Rhodotorula mucilaginosa and Trichosporon jirovecii: A Case Report. Mycopathologia. 2017; 183(3):637-644. PMC: 5958165. DOI: 10.1007/s11046-017-0227-4. View

12.

Costa A, Sidrim J, Cordeiro R, Brilhante R, Monteiro A, Rocha M . Urban pigeons (Columba livia) as a potential source of pathogenic yeasts: a focus on antifungal susceptibility of Cryptococcus strains in Northeast Brazil. Mycopathologia. 2009; 169(3):207-13. DOI: 10.1007/s11046-009-9245-1. View

13.

Lanzafame M, De Checchi G, Parinello A, Trevenzoli M, Cattelan A . Rhodotorula glutinis-related meningitis. J Clin Microbiol. 2001; 39(1):410. PMC: 87748. DOI: 10.1128/JCM.39.1.410.2001. View

14.

Chee H, Lee K . Isolation of Cryptococcus neoformans var. grubii (serotype A) from pigeon droppings in Seoul, Korea. J Microbiol. 2005; 43(5):469-72. View

15.

Lee W, Fong J, Eimes J, Lim Y . Diversity and abundance of human-pathogenic fungi associated with pigeon faeces in urban environments. Mol Ecol. 2017; 26(17):4574-4585. DOI: 10.1111/mec.14216. View

16.

Simi W, Leite-Jr D, Paula C, Hoffmann-Santos H, Takahara D, Hahn R . Yeasts and filamentous fungi in psittacidae and birds of prey droppings in midwest region of Brazil: a potential hazard to human health. Braz J Biol. 2018; 79(3):414-422. DOI: 10.1590/1519-6984.181192. View

17.

Karnik K, Reichle J, Fischetti A, Goggin J . Computed tomographic findings of fungal rhinitis and sinusitis in cats. Vet Radiol Ultrasound. 2009; 50(1):65-8. DOI: 10.1111/j.1740-8261.2008.01491.x. View

18.

Cafarchia C, Camarda A, Romito D, Campolo M, Quaglia N, Tullio D . Occurrence of yeasts in cloacae of migratory birds. Mycopathologia. 2006; 161(4):229-34. DOI: 10.1007/s11046-005-0194-z. View

19.

Chagas-Neto T, Chaves G, Melo A, Colombo A . Bloodstream infections due to Trichosporon spp.: species distribution, Trichosporon asahii genotypes determined on the basis of ribosomal DNA intergenic spacer 1 sequencing, and antifungal susceptibility testing. J Clin Microbiol. 2009; 47(4):1074-81. PMC: 2668300. DOI: 10.1128/JCM.01614-08. View

20.

Kaore N, Atul A, Khan M, Ramnani V . A rare case of human mycosis by Rhizoctonia solani. Indian J Med Microbiol. 2012; 30(3):361-3. DOI: 10.4103/0255-0857.99508. View