Improved Enzymatic in Vitro Reactions in the Gapped Duplex DNA Approach to Oligonucleotide-directed Construction of Mutations

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1988 Jul 25

PMID 3165526

Citations 2

Authors

W Kramer

A Ohmayer

H J Fritz

Affiliations

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Citing Articles

Display of passenger proteins on the surface of Escherichia coli K-12 by the enterohemorrhagic E. coli intimin EaeA.

Wentzel A, Christmann A, Adams T, Kolmar H J Bacteriol. 2001; 183(24):7273-84.

PMID: 11717287 PMC: 95577. DOI: 10.1128/JB.183.24.7273-7284.2001.

Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers.

Stanssens P, Opsomer C, McKeown Y, Kramer W, Zabeau M, Fritz H Nucleic Acids Res. 1989; 17(12):4441-54.

PMID: 2501754 PMC: 318004. DOI: 10.1093/nar/17.12.4441.

References

Craik C, Largman C, Fletcher T, Roczniak S, Barr P, Fletterick R . Redesigning trypsin: alteration of substrate specificity. Science. 1985; 228(4697):291-7. DOI: 10.1126/science.3838593. View

Kramer W, Drutsa V, Jansen H, Kramer B, PFLUGFELDER M, Fritz H . The gapped duplex DNA approach to oligonucleotide-directed mutation construction. Nucleic Acids Res. 1984; 12(24):9441-56. PMC: 320472. DOI: 10.1093/nar/12.24.9441. View