First Molecular Detection of Francisella-like Endosymbionts in Hyalomma and Rhipicephalus Tick Species Collected from Vertebrate Hosts from Sardinia Island, Italy

Overview

Journal Exp Appl Acarol

Specialties Biology
Parasitology

Date 2019 Oct 26

PMID 31650337

Citations 1

Authors

Valentina Chisu

Cipriano Foxi

Giovanna Masala

Affiliations

Soon will be listed here.

Abstract

Ticks are vectors of a wide variety of human and animal pathogens as well as non-pathogenic microorganisms acting as endosymbionts and whose role in ticks is still little known. Symbionts such as Francisella-like endosymbionts (FLEs) are members of Francisellaceae family with unknown pathogenicity, detected in both hard and soft ticks. A total of 236 ticks collected from several sites in Sardinia were screened for Francisella species by PCR using primers targeting a fragment of the 16S rRNA gene. DNA of Francisella was detected in 5.1% (12/236) of the ticks tested. Sequencing results revealed that seven Rhipicephalus sanguineus s.l., three Hyalomma marginatum, one Hy. lusitanicum, and one Rh. bursa ticks exhibited DNA with 99-100% similarity to Francisella-like endosymbionts isolated from different tick species all over the world. Further research is needed in order to better characterize FLE strains obtained in Sardinia and to better understand if their presence could be related to the infection with other zoonotic pathogens.

Citing Articles

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References

Gerhart J, Moses A, Raghavan R . A Francisella-like endosymbiont in the Gulf Coast tick evolved from a mammalian pathogen. Sci Rep. 2016; 6:33670. PMC: 5028885. DOI: 10.1038/srep33670. View

Kimura M . A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980; 16(2):111-20. DOI: 10.1007/BF01731581. View

Szigeti A, Kreizinger Z, Hornok S, Abichu G, Gyuranecz M . Detection of Francisella-like endosymbiont in Hyalomma rufipes from Ethiopia. Ticks Tick Borne Dis. 2014; 5(6):818-20. DOI: 10.1016/j.ttbdis.2014.06.002. View

Chisu V, Foxi C, Mannu R, Satta G, Masala G . A five-year survey of tick species and identification of tick-borne bacteria in Sardinia, Italy. Ticks Tick Borne Dis. 2018; 9(3):678-681. DOI: 10.1016/j.ttbdis.2018.02.008. View

Pistone D, Pajoro M, Novakova E, Vicari N, Gaiardelli C, Vigano R . Ticks and bacterial tick-borne pathogens in Piemonte region, Northwest Italy. Exp Appl Acarol. 2017; 73(3-4):477-491. DOI: 10.1007/s10493-017-0202-2. View

Spitalska E, Sparagano O, Stanko M, Schwarzova K, Spitalsky Z, Skultety L . Diversity of Coxiella-like and Francisella-like endosymbionts, and Rickettsia spp., Coxiella burnetii as pathogens in the tick populations of Slovakia, Central Europe. Ticks Tick Borne Dis. 2018; 9(5):1207-1211. DOI: 10.1016/j.ttbdis.2018.05.002. View

Ebani V, Rocchigiani G, Bertelloni F, Nardoni S, Leoni A, Nicoloso S . Molecular survey on the presence of zoonotic arthropod-borne pathogens in wild red deer (Cervus elaphus). Comp Immunol Microbiol Infect Dis. 2016; 47:77-80. DOI: 10.1016/j.cimid.2016.06.003. View

Gall C, Reif K, Scoles G, Mason K, Mousel M, Noh S . The bacterial microbiome of Dermacentor andersoni ticks influences pathogen susceptibility. ISME J. 2016; 10(8):1846-55. PMC: 5029153. DOI: 10.1038/ismej.2015.266. View

Wang Y, Mao L, Sun Y, Wang Z, Zhang J, Zhang J . A Novel Francisella-Like Endosymbiont in Haemaphysalis longicornis and Hyalomma asiaticum, China. Vector Borne Zoonotic Dis. 2018; 18(12):669-676. DOI: 10.1089/vbz.2017.2252. View

10.

Barns S, Grow C, Okinaka R, Keim P, Kuske C . Detection of diverse new Francisella-like bacteria in environmental samples. Appl Environ Microbiol. 2005; 71(9):5494-500. PMC: 1214603. DOI: 10.1128/AEM.71.9.5494-5500.2005. View

11.

Azagi T, Klement E, Perlman G, Lustig Y, Mumcuoglu K, Apanaskevich D . Francisella-Like Endosymbionts and Rickettsia Species in Local and Imported Hyalomma Ticks. Appl Environ Microbiol. 2017; 83(18). PMC: 5583492. DOI: 10.1128/AEM.01302-17. View

12.

Greay T, Gofton A, Paparini A, Ryan U, Oskam C, Irwin P . Recent insights into the tick microbiome gained through next-generation sequencing. Parasit Vectors. 2018; 11(1):12. PMC: 5755153. DOI: 10.1186/s13071-017-2550-5. View

13.

Liu L, Liu J, Liu Z, Yu Z, Xu S, Yang X . Microbial communities and symbionts in the hard tick Haemaphysalis longicornis (Acari: Ixodidae) from north China. Parasit Vectors. 2014; 6(1):310. PMC: 3813991. DOI: 10.1186/1756-3305-6-310. View

14.

Pajoro M, Pistone D, Varotto Boccazzi I, Mereghetti V, Bandi C, Fabbi M . Molecular screening for bacterial pathogens in ticks (Ixodes ricinus) collected on migratory birds captured in northern Italy. Folia Parasitol (Praha). 2018; 65. DOI: 10.14411/fp.2018.008. View

15.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S . MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013; 30(12):2725-9. PMC: 3840312. DOI: 10.1093/molbev/mst197. View

16.

de la Fuente J, Antunes S, Bonnet S, Cabezas-Cruz A, Domingos A, Estrada-Pena A . Tick-Pathogen Interactions and Vector Competence: Identification of Molecular Drivers for Tick-Borne Diseases. Front Cell Infect Microbiol. 2017; 7:114. PMC: 5383669. DOI: 10.3389/fcimb.2017.00114. View

17.

Cerutti F, Modesto P, Rizzo F, Cravero A, Jurman I, Costa S . The microbiota of hematophagous ectoparasites collected from migratory birds. PLoS One. 2018; 13(8):e0202270. PMC: 6110481. DOI: 10.1371/journal.pone.0202270. View

18.

Ahantarig A, Trinachartvanit W, Baimai V, Grubhoffer L . Hard ticks and their bacterial endosymbionts (or would be pathogens). Folia Microbiol (Praha). 2013; 58(5):419-28. DOI: 10.1007/s12223-013-0222-1. View

19.

Brinkmann A, Hekimoglu O, Dincer E, Hagedorn P, Nitsche A, Ergunay K . A cross-sectional screening by next-generation sequencing reveals Rickettsia, Coxiella, Francisella, Borrelia, Babesia, Theileria and Hemolivia species in ticks from Anatolia. Parasit Vectors. 2019; 12(1):26. PMC: 6329055. DOI: 10.1186/s13071-018-3277-7. View

20.

Felsenstein J . CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP. Evolution. 2017; 39(4):783-791. DOI: 10.1111/j.1558-5646.1985.tb00420.x. View