Analysis of Corneal Stromal Roughness After IFS 150 KHz and LenSx Femtosecond LASIK Flap Creation in Porcine Eyes

Overview

Journal Graefes Arch Clin Exp Ophthalmol

Specialty Ophthalmology

Date 2019 Oct 26

PMID 31650272

Citations 3

Authors

Juan Gros-Otero

Samira Ketabi

Rafael Canones-Zafra

Montserrat Garcia-Gonzalez

Alberto Parafita-Fernandez

Cesar Villa-Collar

Santiago Casado

Miguel Teus

Affiliations

Soon will be listed here.

Abstract

Purpose: To describe and compare the stromal bed roughness obtained after laser in situ keratomileusis (LASIK) flap creation using a corneal femtosecond laser platform (iFS 150) and a "dual" femtosecond (FS) laser platform (LenSx).

Methods: This ex vivo experimental study was conducted in an animal model using 12 freshly enucleated porcine eyes, six with each femtosecond laser. The standard laser treatment parameters were used for the experiment. After LASIK flap creation, the corneal stromal roughness was assessed using atomic force microscopy (AFM) in contact mode immersed in liquid. In each sample, surface measurements were obtained in 60 regions of six eyes per FS laser in 10 20 × 20-micron areas of the central corneal stroma at 512 × 512-point resolution. The surface roughness was measured and the root-mean-square (RMS) values of the roughness were obtained.

Results: The mean RMS ± standard deviation values were 430 ± 150 nm for the corneal femtosecond laser platform and 370 ± 100 nm for the dual FS laser platform (P < 0.011).

Conclusions: In this experimental study with AFM, we found smoother stromal beds after LASIK flap creation with LenSx compared to iFS 150 kHz. Further studies are needed to understand visual implications of the differences found.

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