Long Non-coding RNA HOTAIR Functions As a Competitive Endogenous RNA to Regulate PRAF2 Expression by Sponging MiR-326 in Cutaneous Squamous Cell Carcinoma

Overview

Journal Cancer Cell Int

Publisher Biomed Central

Date 2019 Oct 26

PMID 31649487

Citations 34

Authors

Guo-Jun Yu

Yong Sun

Da-Wei Zhang

Peng Zhang

Affiliations

Soon will be listed here.

Abstract

Background: LncRNAs may exert a regulatory effect in tumorigenesis. Although the expression of lncRNA has been confirmed to be notably elevated in the tissues of CSCC, its biological mechanism in CSCC is still unknown.

Methods: expression level in CSCC cell lines was monitored via qRT-PCR. Then CCK-8 assay, Transwell assay and EdU assay were adopted to detect cell migration and proliferation. Meanwhile, through bioinformatics analysis and luciferase reporter gene detection, a new target of was identified. Additionally, Western blotting and RIP analysis were adopted to discuss the possible mechanism.

Results: expression in CSCC cell lines exhibited an obvious elevation. Cell function analysis revealed that overexpression remarkably facilitated CSCC cell migration, proliferation and EMT process, which were impeded by down-regulation of . Furthermore, competitively bound to miR-326, so as to positively modulate miR-326 expression.

Conclusions: These results present that , as a ceRNA, regulates expression by competitive binding to miR-326 during CSCC.

Citing Articles

Circulating lncRNA HOTAIR is a biomarker for pediatric acute lymphoblastic leukemia and mediator of miR-326 exosomal export.

Dashti N, Fadavi D, Rezaei R, Rahgozar S, Moafi A Sci Rep. 2025; 15(1):4901.

PMID: 39929912 PMC: 11811015. DOI: 10.1038/s41598-025-87857-0.

Bioinformatics analysis of ceRNA network of autophagy-related genes in pediatric asthma.

Zhu H, Shi J, Li W Medicine (Baltimore). 2023; 102(48):e36343.

PMID: 38050261 PMC: 10695615. DOI: 10.1097/MD.0000000000036343.

Molecular Alterations in Cutaneous Squamous Cell Carcinoma in Immunocompetent and Immunosuppressed Hosts-A Systematic Review.

Tsang D, Tam S, Oh C Cancers (Basel). 2023; 15(6).

PMID: 36980718 PMC: 10046480. DOI: 10.3390/cancers15061832.

The long non-coding RNA PVT1 promotes tumorigenesis of cutaneous squamous cell carcinoma via interaction with 4EBP1.

Li R, Huang D, Ju M, Chen H, Luan C, Zhang J Cell Death Discov. 2023; 9(1):101.

PMID: 36944636 PMC: 10030977. DOI: 10.1038/s41420-023-01380-7.

De novo annotation of lncRNA HOTAIR transcripts by long-read RNA capture-seq reveals a differentiation-driven isoform switch.

Potolitsyna E, Hazell Pickering S, Tooming-Klunderud A, Collas P, Briand N BMC Genomics. 2022; 23(1):658.

PMID: 36115964 PMC: 9482196. DOI: 10.1186/s12864-022-08887-w.

References

Chang L, Guo R, Yuan Z, Shi H, Zhang D . LncRNA HOTAIR Regulates CCND1 and CCND2 Expression by Sponging miR-206 in Ovarian Cancer. Cell Physiol Biochem. 2018; 49(4):1289-1303. DOI: 10.1159/000493408. View

Didona D, Paolino G, Bottoni U, Cantisani C . Non Melanoma Skin Cancer Pathogenesis Overview. Biomedicines. 2018; 6(1). PMC: 5874663. DOI: 10.3390/biomedicines6010006. View

Yesantharao P, Wang W, Ioannidis N, Demehri S, Whittemore A, Asgari M . Cutaneous squamous cell cancer (cSCC) risk and the human leukocyte antigen (HLA) system. Hum Immunol. 2017; 78(4):327-335. PMC: 5428079. DOI: 10.1016/j.humimm.2017.02.002. View

Ghaemi Z, Mohammad Soltani B, Mowla S . MicroRNA-326 Functions as a Tumor Suppressor in Breast Cancer by Targeting ErbB/PI3K Signaling Pathway. Front Oncol. 2019; 9:653. PMC: 6682688. DOI: 10.3389/fonc.2019.00653. View

Xu F, Yang J, Shang J, Lan F, Li M, Shi L . MicroRNA-302d promotes the proliferation of human pluripotent stem cell-derived cardiomyocytes by inhibiting in the Hippo pathway. Clin Sci (Lond). 2019; 133(13):1387-1399. DOI: 10.1042/CS20190099. View

Liu L, Cui S, Wan T, Li X, Tian W, Zhang R . Long non-coding RNA HOTAIR acts as a competing endogenous RNA to promote glioma progression by sponging miR-126-5p. J Cell Physiol. 2018; 233(9):6822-6831. DOI: 10.1002/jcp.26432. View

Wang S, Tran E . Unexpected functions of lncRNAs in gene regulation. Commun Integr Biol. 2014; 6(6):e27610. PMC: 3917965. DOI: 10.4161/cib.27610. View

Mei X, Zhong S . Long noncoding RNA LINC00520 prevents the progression of cutaneous squamous cell carcinoma through the inactivation of the PI3K/Akt signaling pathway by downregulating EGFR. Chin Med J (Engl). 2019; 132(4):454-465. PMC: 6595718. DOI: 10.1097/CM9.0000000000000070. View

Li Y, Gao Y, Xu Y, Ma H, Yang M . Down-regulation of miR-326 is associated with poor prognosis and promotes growth and metastasis by targeting FSCN1 in gastric cancer. Growth Factors. 2015; 33(4):267-74. DOI: 10.3109/08977194.2015.1076406. View

10.

Gao L, Wang X, Guo S, Xiao L, Liang C, Wang Z . LncRNA HOTAIR functions as a competing endogenous RNA to upregulate SIRT1 by sponging miR-34a in diabetic cardiomyopathy. J Cell Physiol. 2018; 234(4):4944-4958. DOI: 10.1002/jcp.27296. View

11.

Dong L, Hui L . HOTAIR Promotes Proliferation, Migration, and Invasion of Ovarian Cancer SKOV3 Cells Through Regulating PIK3R3. Med Sci Monit. 2016; 22:325-31. PMC: 4750754. DOI: 10.12659/msm.894913. View

12.

Zhou T, Ding J, Wang X, Zheng X . Long noncoding RNAs and atherosclerosis. Atherosclerosis. 2016; 248:51-61. DOI: 10.1016/j.atherosclerosis.2016.02.025. View

13.

Pardo P, Hajira A, Boriek A, Mohamed J . MicroRNA-434-3p regulates age-related apoptosis through eIF5A1 in the skeletal muscle. Aging (Albany NY). 2017; 9(3):1012-1029. PMC: 5391215. DOI: 10.18632/aging.101207. View

14.

Mao Z, Li H, Du B, Cui K, Xing Y, Zhao X . LncRNA DANCR promotes migration and invasion through suppression of lncRNA-LET in gastric cancer cells. Biosci Rep. 2017; 37(6). PMC: 5672085. DOI: 10.1042/BSR20171070. View

15.

Bergmann J, Spector D . Long non-coding RNAs: modulators of nuclear structure and function. Curr Opin Cell Biol. 2014; 26:10-8. PMC: 3927160. DOI: 10.1016/j.ceb.2013.08.005. View

16.

Borsics T, Lundberg E, Geerts D, Koomoa D, Koster J, Wester K . Subcellular distribution and expression of prenylated Rab acceptor 1 domain family, member 2 (PRAF2) in malignant glioma: Influence on cell survival and migration. Cancer Sci. 2010; 101(7):1624-31. PMC: 11158841. DOI: 10.1111/j.1349-7006.2010.01570.x. View

17.

Yu Y, Lv F, Liang D, Yang Q, Zhang B, Lin H . HOTAIR may regulate proliferation, apoptosis, migration and invasion of MCF-7 cells through regulating the P53/Akt/JNK signaling pathway. Biomed Pharmacother. 2017; 90:555-561. DOI: 10.1016/j.biopha.2017.03.054. View

18.

Sand M, Gambichler T, Sand D, Skrygan M, Altmeyer P, Bechara F . MicroRNAs and the skin: tiny players in the body's largest organ. J Dermatol Sci. 2008; 53(3):169-75. DOI: 10.1016/j.jdermsci.2008.10.004. View

19.

Liu-Smith F, Jia J, Zheng Y . UV-Induced Molecular Signaling Differences in Melanoma and Non-melanoma Skin Cancer. Adv Exp Med Biol. 2017; 996:27-40. DOI: 10.1007/978-3-319-56017-5_3. View

20.

Yanez D, Lacher R, Vidyarthi A, Colegio O . The role of macrophages in skin homeostasis. Pflugers Arch. 2017; 469(3-4):455-463. PMC: 5663320. DOI: 10.1007/s00424-017-1953-7. View