3D Image Analysis Reveals Differences of CD30 Positive Cells and Network Formation in Reactive and Malignant Human Lymphoid Tissue (classical Hodgkin Lymphoma)

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2019 Oct 25

PMID 31648255

Citations 5

Authors

Julia Liebers

Patrick Wurzel

Kerstin Bianca Reisinger

Martin-Leo Hansmann

Affiliations

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Abstract

Aims: The examination of histological sections is still the gold standard in diagnostic pathology. Important histopathological diagnostic criteria are nuclear shapes and chromatin distribution as well as nucleus-cytoplasm relation and immunohistochemical properties of surface and intracellular proteins. The aim of this investigation was to evaluate the benefits and drawbacks of three-dimensional imaging of CD30+ cells in classical Hodgkin Lymphoma (cHL) in comparison to CD30+ lymphoid cells in reactive lymphoid tissues.

Materials And Results: Using immunoflourescence confocal microscopy and computer-based analysis, we compared CD30+ neoplastic cells in Nodular Sclerosis cHL (NScCHL), Mixed Cellularity cHL (MCcHL), with reactive CD30+ cells in Adenoids (AD) and Lymphadenitis (LAD). We confirmed that the percentage of CD30+ cell volume can be calculated. The amount in lymphadenitis was approx. 1.5%, in adenoids around 2%, in MCcHL up to 4,5% whereas the values for NScHL rose to more than 8% of the total cell cytoplasm. In addition, CD30+ tumour cells (HRS-cells) in cHL had larger volumes, and more protrusions compared to CD30+ reactive cells. Furthermore, the formation of large cell networks turned out to be a typical characteristic of NScHL.

Conclusion: In contrast to 2D histology, 3D laser scanning offers a visualisation of complete cells, their network interaction and spatial distribution in the tissue. The possibility to differentiate cells in regards to volume, surface, shape, and cluster formation enables a new view on further diagnostic and biological questions. 3D includes an increased amount of information as a basis of bioinformatical calculations.

Citing Articles

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Hansmann M Pathologie (Heidelb). 2023; 44(Suppl 3):140-143.

PMID: 38010387 DOI: 10.1007/s00292-023-01265-7.

Imaging bridges pathology and radiology.

Martin-Leo H, Frederick K, Wojciech S, Klaus-Robert M, Emmanuel D, Sonja S J Pathol Inform. 2023; 14:100298.

PMID: 36851923 PMC: 9958472. DOI: 10.1016/j.jpi.2023.100298.

T-cell-derived Hodgkin lymphoma has motility characteristics intermediate between Hodgkin and anaplastic large cell lymphoma.

Bein J, Flinner N, Haupl B, Mathur A, Schneider O, Abu-Ayyad M J Cell Mol Med. 2022; 26(12):3495-3505.

PMID: 35586951 PMC: 9189347. DOI: 10.1111/jcmm.17389.

3D analyses reveal T cells with activated nuclear features in T-cell/histiocyte-rich large B-cell lymphoma.

Sadeghi Shoreh Deli A, Scharf S, Steiner Y, Bein J, Hansmann M, Hartmann S Mod Pathol. 2022; 35(10):1431-1438.

PMID: 35173297 PMC: 9514992. DOI: 10.1038/s41379-022-01016-8.

3D investigation shows walls and wall-like structures around human germinal centres, probably regulating T- and B-cell entry and exit.

Thomos M, Wurzel P, Scharf S, Koch I, Hansmann M PLoS One. 2020; 15(11):e0242177.

PMID: 33170900 PMC: 7654765. DOI: 10.1371/journal.pone.0242177.

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