Functional Human CD141+ Dendritic Cells in Human Immune System Mice

Overview

Journal J Infect Dis

Publisher Oxford University Press

Specialty Infectious Diseases

Date 2019 Oct 25

PMID 31647546

Citations 4

Authors

Jordana G A Coelho-Dos-Reis

Ryota Funakoshi

Jing Huang

Felipe Valenca Pereira

Sho Iketani

Moriya Tsuji

Affiliations

Soon will be listed here.

Abstract

Background: For the purpose of studying functional human dendritic cells (DCs) in a humanized mouse model that mimics the human immune system (HIS), a model referred to as HIS mice was established.

Methods: Human immune system mice were made by engrafting NOD/SCID/IL2Rgammanull (NSG) mice with human hematopoietic stem cells (HSCs) following the transduction of genes encoding human cytokines and human leukocyte antigen (HLA)-A2.1 by adeno-associated virus serotype 9 (AAV9) vectors.

Results: Our results indicate that human DC subsets, such as CD141+CD11c+ and CD1c+CD11c+ myeloid DCs, distribute throughout several organs in HIS mice including blood, bone marrow, spleen, and draining lymph nodes. The CD141+CD11c+ and CD1c+CD11c+ human DCs isolated from HIS mice immunized with adenoviruses expressing malaria/human immunodeficiency virus (HIV) epitopes were able to induce the proliferation of malaria/HIV epitopes-specific human CD8+ T cells in vitro. Upregulation of CD1c was also observed in human CD141+ DCs 1 day after immunization with the adenovirus-based vaccines.

Conclusions: Establishment of such a humanized mouse model that mounts functional human DCs enables preclinical assessment of the immunogenicity of human vaccines in vivo.

Citing Articles

A Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse Strains.

Gutierrez-Barbosa H, Medina-Moreno S, Perdomo-Celis F, Davis H, Coronel-Ruiz C, Zapata J Microorganisms. 2023; 11(6).

PMID: 37375051 PMC: 10300940. DOI: 10.3390/microorganisms11061548.

Viral delivery of a peptide-based immunomodulator enhances T cell priming during vaccination.

Phares T, Huang J, Kotraiah V, Hauser M, Domi A, Oruganti S Front Pharmacol. 2022; 13:1029636.

PMID: 36582528 PMC: 9792674. DOI: 10.3389/fphar.2022.1029636.

An In Vivo Screen to Identify Short Peptide Mimotopes with Enhanced Antitumor Immunogenicity.

He X, Zhou S, Quinn B, Jahagirdar D, Ortega J, Long M Cancer Immunol Res. 2022; 10(3):314-326.

PMID: 34992135 PMC: 8898291. DOI: 10.1158/2326-6066.CIR-21-0332.

Targeted Co-delivery of Tumor Antigen and α-Galactosylceramide to CD141 Dendritic Cells Induces a Potent Tumor Antigen-Specific Human CD8 T Cell Response in Human Immune System Mice.

Huang J, Zhou J, Ghinnagow R, Seki T, Iketani S, Soulard D Front Immunol. 2020; 11:2043.

PMID: 32973811 PMC: 7461784. DOI: 10.3389/fimmu.2020.02043.

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