A Unicellular Relative of Animals Generates a Layer of Polarized Cells by Actomyosin-dependent Cellularization

Overview

Journal Elife

Specialty Biology

Date 2019 Oct 25

PMID 31647412

Citations 26

Authors

Omaya Dudin

Andrej Ondracka

Xavier Grau-Bove

Arthur Ab Haraldsen

Atsushi Toyoda

Hiroshi Suga

Jon Brate

Inaki Ruiz-Trillo

Affiliations

Soon will be listed here.

Abstract

In animals, cellularization of a coenocyte is a specialized form of cytokinesis that results in the formation of a polarized epithelium during early embryonic development. It is characterized by coordinated assembly of an actomyosin network, which drives inward membrane invaginations. However, whether coordinated cellularization driven by membrane invagination exists outside animals is not known. To that end, we investigate cellularization in the ichthyosporean , a close unicellular relative of animals. We show that the process of cellularization involves coordinated inward plasma membrane invaginations dependent on an actomyosin network and reveal the temporal order of its assembly. This leads to the formation of a polarized layer of cells resembling an epithelium. We show that this stage is associated with tightly regulated transcriptional activation of genes involved in cell adhesion. Hereby we demonstrate the presence of a self-organized, clonally-generated, polarized layer of cells in a unicellular relative of animals.

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