Immunohistochemical Expression of Plasminogen Activator Inhibitor-1 in Subcutaneous Versus Omental Adipose Tissue in Patients After Elective Abdominal Surgery

Overview

Journal Autops Case Rep

Date 2019 Oct 24

PMID 31641662

Citations 1

Authors

Mehmet Yildiz

Emine Bozkurtlar

Abdulmunir Azizy

Mehmet Agirbasli

Affiliations

Soon will be listed here.

Abstract

Plasminogen activator inhibitor-1 (PAI-1) is a biomarker of thrombosis. Adipose and vascular tissues are among the major sources of PAI-1 production. Previous studies indicated that fat deposits mediate increased cardiovascular risk among obese individuals. We investigated the immunohistochemical (IHC) expression of PAI-1 in adipose and vascular tissues from the omentum and the subcutaneous tissue. The pathology samples were selected from 37 random patients who underwent elective abdominal surgery between 2008-2009. PAI-1 expression was semi-quantitatively scored and compared between the groups. Significant differences were noted in the IHC expression of PAI-1 between the omental and the subcutaneous adipose tissues (1.1 ± 0.8 versus 0.8 ± 0.6, respectively (p=0.05)). Adipose tissue displayed higher IHC expression of PAI-1 compared to vascular wall tissue in both omentum and subcutaneous sections (1.1 ± 0.8 versus 0.5 ± 0.9 (p=0.004), and 0.8 ± 0.6 versus 0.4 ± 0.6 (p=0.003), respectively). In conclusion, our study compared PAI-1 expression in the omentum versus the subcutaneous tissue and adipose versus vascular tissues. IHC expression of PAI-1 level was significantly higher in the omental adipose tissue compared to the subcutaneous adipose tissue. Adipose tissue displayed significantly higher PAI-1 expression than vascular tissue. The study elucidates the biological differences of adipose and vascular tissue from subcutaneous versus omental sections.

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References

Abraham T, Pedley A, Massaro J, Hoffmann U, Fox C . Association between visceral and subcutaneous adipose depots and incident cardiovascular disease risk factors. Circulation. 2015; 132(17):1639-47. PMC: 4779497. DOI: 10.1161/CIRCULATIONAHA.114.015000. View

Poirier P, Despres J . Waist circumference, visceral obesity, and cardiovascular risk. J Cardiopulm Rehabil. 2003; 23(3):161-9. DOI: 10.1097/00008483-200305000-00001. View

Cigolini M, Targher G, Bergamo Andreis I, Tonoli M, AGOSTINO G, De Sandre G . Visceral fat accumulation and its relation to plasma hemostatic factors in healthy men. Arterioscler Thromb Vasc Biol. 1996; 16(3):368-74. DOI: 10.1161/01.atv.16.3.368. View

Eriksson P, van Harmelen V, Hoffstedt J, Lundquist P, Vidal H, Stemme V . Regional variation in plasminogen activator inhibitor-1 expression in adipose tissue from obese individuals. Thromb Haemost. 2000; 83(4):545-8. View

OConnell J, Lynch L, Cawood T, Kwasnik A, Nolan N, Geoghegan J . The relationship of omental and subcutaneous adipocyte size to metabolic disease in severe obesity. PLoS One. 2010; 5(4):e9997. PMC: 2848665. DOI: 10.1371/journal.pone.0009997. View

Birgel M, Rohrig K, Hauner H . Effect of tumor necrosis factor alpha and transforming growth factor beta 1 on plasminogen activator inhibitor-1 secretion from subcutaneous and omental human fat cells in suspension culture. Metabolism. 2000; 49(5):666-71. DOI: 10.1016/s0026-0495(00)80046-3. View

Giltay E, Elbers J, Gooren L, Emeis J, Kooistra T, Asscheman H . Visceral fat accumulation is an important determinant of PAI-1 levels in young, nonobese men and women: modulation by cross-sex hormone administration. Arterioscler Thromb Vasc Biol. 1998; 18(11):1716-22. DOI: 10.1161/01.atv.18.11.1716. View

Cesari M, Pahor M, Antonelli Incalzi R . Plasminogen activator inhibitor-1 (PAI-1): a key factor linking fibrinolysis and age-related subclinical and clinical conditions. Cardiovasc Ther. 2010; 28(5):e72-91. PMC: 2958211. DOI: 10.1111/j.1755-5922.2010.00171.x. View

Harman-Boehm I, Bluher M, Redel H, Sion-Vardy N, Ovadia S, Avinoach E . Macrophage infiltration into omental versus subcutaneous fat across different populations: effect of regional adiposity and the comorbidities of obesity. J Clin Endocrinol Metab. 2007; 92(6):2240-7. DOI: 10.1210/jc.2006-1811. View

10.

Kolev K, Machovich R . Molecular and cellular modulation of fibrinolysis. Thromb Haemost. 2003; 89(4):610-21. View

11.

Barnard S, Pieters M, de Lange Z . The contribution of different adipose tissue depots to plasma plasminogen activator inhibitor-1 (PAI-1) levels. Blood Rev. 2016; 30(6):421-429. DOI: 10.1016/j.blre.2016.05.002. View

12.

Landin K, Stigendal L, Eriksson E, Krotkiewski M, Risberg B, Tengborn L . Abdominal obesity is associated with an impaired fibrinolytic activity and elevated plasminogen activator inhibitor-1. Metabolism. 1990; 39(10):1044-8. DOI: 10.1016/0026-0495(90)90164-8. View

13.

Cigolini M, Tonoli M, Borgato L, Frigotto L, Manzato F, Zeminian S . Expression of plasminogen activator inhibitor-1 in human adipose tissue: a role for TNF-alpha?. Atherosclerosis. 1999; 143(1):81-90. DOI: 10.1016/s0021-9150(98)00281-0. View

14.

Agirbasli M . Pivotal role of plasminogen-activator inhibitor 1 in vascular disease. Int J Clin Pract. 2005; 59(1):102-6. DOI: 10.1111/j.1742-1241.2005.00379.x. View

15.

Vaughan D . PAI-1 and TGF-beta: unmasking the real driver of TGF-beta-induced vascular pathology. Arterioscler Thromb Vasc Biol. 2006; 26(4):679-80. DOI: 10.1161/01.ATV.0000209949.86606.c2. View

16.

Mertens I, Van Gaal L . Visceral fat as a determinant of fibrinolysis and hemostasis. Semin Vasc Med. 2005; 5(1):48-55. DOI: 10.1055/s-2005-871741. View

17.

Bastelica D, Morange P, Berthet B, Borghi H, Lacroix O, Grino M . Stromal cells are the main plasminogen activator inhibitor-1-producing cells in human fat: evidence of differences between visceral and subcutaneous deposits. Arterioscler Thromb Vasc Biol. 2002; 22(1):173-8. DOI: 10.1161/hq0102.101552. View

18.

Yang Y, Chen M, Clements R, Abrams G, Aprahamian C, Harmon C . Human mesenteric adipose tissue plays unique role versus subcutaneous and omental fat in obesity related diabetes. Cell Physiol Biochem. 2008; 22(5-6):531-8. DOI: 10.1159/000185527. View

19.

Tsantes A, Nikolopoulos G, Bagos P, Bonovas S, Kopterides P, Vaiopoulos G . The effect of the plasminogen activator inhibitor-1 4G/5G polymorphism on the thrombotic risk. Thromb Res. 2007; 122(6):736-42. DOI: 10.1016/j.thromres.2007.09.005. View

20.

Fox C, Massaro J, Hoffmann U, Pou K, Maurovich-Horvat P, Liu C . Abdominal visceral and subcutaneous adipose tissue compartments: association with metabolic risk factors in the Framingham Heart Study. Circulation. 2007; 116(1):39-48. DOI: 10.1161/CIRCULATIONAHA.106.675355. View