Effect of the PH in the Enrichment of X or Y Sex Chromosome-bearing Sperm in Bovine

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2019 Oct 24

PMID 31641311

Citations 4

Authors

Nidhi P Raval

Tejas M Shah

Linz-Buoy George

Chaitanya G Joshi

Affiliations

Soon will be listed here.

Abstract

Background And Aim: Studies have shown that the pH of the vagina during the course of fertilization may influence the migration of X- and Y-bearing spermatozoa and thus leading to skewness in the sex of the offspring. Hence, this study was carried out to check the effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine ().

Materials And Methods: To check the effect of pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine, we used buffers of various pH ranging from 5.5 to 9.0 for swim-up procedure of sperm sample and collected upper and bottom fraction from the same buffer and checked the abundance of X- and Y-bearing spermatozoa by droplet digital polymerase chain reaction using X- and Y-chromosome-specific DNA probe.

Results: The abundance of X- and Y-bearing spermatozoa was not differed significantly in either of the fraction collected.

Conclusion: Thus, it appears to be unlikely that an immediate impact of pH on sperm can be a solitary impact on the sex of offspring in bovine.

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