Genetically Related Strains Displayed Differential Intracellular Growth in Bovine Macrophages

Overview

Journal Vet Sci

Publisher MDPI

Specialty Veterinary Medicine

Date 2019 Oct 23

PMID 31635257

Citations 1

Authors

Alejandro Benitez-Guzman

Hugo Esquivel-Solis

Cecilia Romero-Torres

Camila Arriaga-Diaz

Jose A Gutierrez-Pabello

Affiliations

Soon will be listed here.

Abstract

Molecular typing of bacterial isolates provides a powerful approach for distinguishing genotypes. It is known that strain virulence plays a role in prevalence and spread of the disease, suggesting that strain virulence and prevailing genotypes are associated. However, it is not well understood whether strain virulence correlates with particular genotypes. In this study, we assessed the in vitro intracellular growth of 18 isolates in bovine macrophages as an indicator of bacterial virulence and sought a relationship with the genotype identified by spoligotyping. We found 14 different spoligotypes-11 were already known and three spoligotypes had never been reported before. We identified 2 clusters that were phylogenetically related, containing 10 and 6 strains, respectively, and 2 orphan strains. Intracellular growth and phagocytic rates of 18 strains were heterogeneous. Our results suggest that intracellular growth and phagocytosis are independent of the bacterial lineage identified by spoligotyping.

Citing Articles

Antimycobacterial Potential of Mycobacteriophage Under Disease-Mimicking Conditions.

Kalapala Y, Sharma P, Agarwal R Front Microbiol. 2020; 11:583661.

PMID: 33381088 PMC: 7767895. DOI: 10.3389/fmicb.2020.583661.

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