Molecular Mapping of a Novel Male-sterile Gene Ms in Soybean [Glycine Max (L.) Merr.]

Overview

Journal Plant Reprod

Publisher Springer

Specialty Biology

Date 2019 Oct 18

PMID 31620875

Citations 12

Authors

Zhixing Nie

Tuanjie Zhao

Meifeng Liu

Jinying Dai

Tingting He

Duo Lyu

Jinming Zhao

Shouping Yang

Junyi Gai

Affiliations

Soon will be listed here.

Abstract

Nuclear male sterility (NMS) is a potential characteristic in crop recurrent selection and hybrid breeding. Mapping of nuclear male-sterile genes is key to utilizing NMS. Previously, we discovered a spontaneous soybean (Glycine max [L.] Merr.) male-sterile female-fertile mutant NJS-13H, which was conferred by a single recessive gene, designated ms. In this study, the ms was mapped to Chromosome 10 (LG O), and narrowed down between two SSR (simple sequence repeats) markers, BARCSOYSSR_10_794 and BARCSOYSSR_10_819 using three heterozygote-derived segregating populations, i.e., (NJS-13H × NN1138-2)F, (NJS-13H × N2899)F and (NJS-13H)SPAG (segregating populations in advanced generations). This region spans approximately 1.32 Mb, where 27 genes were annotated according to the soybean reference genome sequence (Wm82.a2.v1). Among them, four genes were recognized as candidate genes for ms. Comparing to the physical locations of all the known male-sterile loci, ms is demonstrated to be a new male-sterile locus. This result may help the utilization and cloning of the gene.

Citing Articles

Transcriptome and WGCNA reveals the potential genetic basis of photoperiod-sensitive male sterility in soybean.

Yang Y, He S, Xu L, Wang M, Chen S, Bai Z BMC Genomics. 2025; 26(1):131.

PMID: 39934659 PMC: 11816801. DOI: 10.1186/s12864-025-11314-5.

MS2/GmAMS1 encodes a bHLH transcription factor important for tapetum degeneration in soybean.

Yu J, Xu Y, Huang Y, Zhu Y, Zhou L, Zhang Y Plant Cell Rep. 2024; 43(9):211.

PMID: 39127985 DOI: 10.1007/s00299-024-03300-0.

Deciphering the roles of unknown/uncharacterized genes in plant development and stress responses.

Wang X, Wang B, Yuan F Front Plant Sci. 2023; 14:1276559.

PMID: 38078098 PMC: 10701545. DOI: 10.3389/fpls.2023.1276559.

The Assessment of Natural Cross Pollination Properties of a Novel Male-Sterile-Female-Fertile Mutation in Soybean.

Wang W, Zhu X, Zhang Y, Gao H, Zhang Z, Yang C Plants (Basel). 2023; 12(20).

PMID: 37896002 PMC: 10610278. DOI: 10.3390/plants12203538.

Male sterility and hybrid breeding in soybean.

Fang X, Sun Y, Li J, Li M, Zhang C Mol Breed. 2023; 43(6):47.

PMID: 37309310 PMC: 10248680. DOI: 10.1007/s11032-023-01390-4.

References

Hwang E, Song Q, Jia G, Specht J, Hyten D, Costa J . A genome-wide association study of seed protein and oil content in soybean. BMC Genomics. 2014; 15:1. PMC: 3890527. DOI: 10.1186/1471-2164-15-1. View

Han M, Jung K, Yi G, Lee D, An G . Rice Immature Pollen 1 (RIP1) is a regulator of late pollen development. Plant Cell Physiol. 2006; 47(11):1457-72. DOI: 10.1093/pcp/pcl013. View

Zhang Q, Shen B, Dai X, Mei M, Saghai Maroof M, Li Z . Using bulked extremes and recessive class to map genes for photoperiod-sensitive genic male sterility in rice. Proc Natl Acad Sci U S A. 1994; 91(18):8675-9. PMC: 44669. DOI: 10.1073/pnas.91.18.8675. View

Cervantes-Martinez I, Sandhu D, Xu M, Ortiz-Perez E, Kato K, Horner H . The male sterility locus ms3 is present in a fertility controlling gene cluster in soybean. J Hered. 2009; 100(5):565-70. DOI: 10.1093/jhered/esp054. View

Liu R, Meng J . [MapDraw: a microsoft excel macro for drawing genetic linkage maps based on given genetic linkage data]. Yi Chuan. 2005; 25(3):317-21. View

Yi B, Chen Y, Lei S, Tu J, Fu T . Fine mapping of the recessive genic male-sterile gene (Bnms1) in Brassica napus L. Theor Appl Genet. 2006; 113(4):643-50. DOI: 10.1007/s00122-006-0328-9. View

Yang Y, Speth B, Boonyoo N, Baumert E, Atkinson T, Palmer R . Molecular mapping of three male-sterile, female-fertile mutants and generation of a comprehensive map of all known male sterility genes in soybean. Genome. 2014; 57(3):155-60. DOI: 10.1139/gen-2014-0018. View

Du J, Tian Z, Sui Y, Zhao M, Song Q, Cannon S . Pericentromeric effects shape the patterns of divergence, retention, and expression of duplicated genes in the paleopolyploid soybean. Plant Cell. 2012; 24(1):21-32. PMC: 3289580. DOI: 10.1105/tpc.111.092759. View

Zhang H, Liang W, Yang X, Luo X, Jiang N, Ma H . Carbon starved anther encodes a MYB domain protein that regulates sugar partitioning required for rice pollen development. Plant Cell. 2010; 22(3):672-89. PMC: 2861464. DOI: 10.1105/tpc.109.073668. View

10.

Frasch R, Weigand C, Perez P, Palmer R, Sandhu D . Molecular mapping of 2 environmentally sensitive male-sterile mutants in soybean. J Hered. 2010; 102(1):11-6. DOI: 10.1093/jhered/esq100. View

11.

Lander E, Green P, ABRAHAMSON J, Barlow A, Daly M, Lincoln S . MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987; 1(2):174-81. DOI: 10.1016/0888-7543(87)90010-3. View

12.

Li H, Pinot F, Sauveplane V, Werck-Reichhart D, Diehl P, Schreiber L . Cytochrome P450 family member CYP704B2 catalyzes the {omega}-hydroxylation of fatty acids and is required for anther cutin biosynthesis and pollen exine formation in rice. Plant Cell. 2010; 22(1):173-90. PMC: 2828706. DOI: 10.1105/tpc.109.070326. View

13.

Song Q, Marek L, Shoemaker R, Lark K, Concibido V, Delannay X . A new integrated genetic linkage map of the soybean. Theor Appl Genet. 2004; 109(1):122-8. DOI: 10.1007/s00122-004-1602-3. View

14.

Zhou S, Wang Y, Li W, Zhao Z, Ren Y, Wang Y . Pollen semi-sterility1 encodes a kinesin-1-like protein important for male meiosis, anther dehiscence, and fertility in rice. Plant Cell. 2011; 23(1):111-29. PMC: 3051251. DOI: 10.1105/tpc.109.073692. View

15.

Li N, Zhang D, Liu H, Yin C, Li X, Liang W . The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development. Plant Cell. 2006; 18(11):2999-3014. PMC: 1693939. DOI: 10.1105/tpc.106.044107. View

16.

Liu Z, Bao W, Liang W, Yin J, Zhang D . Identification of gamyb-4 and analysis of the regulatory role of GAMYB in rice anther development. J Integr Plant Biol. 2010; 52(7):670-8. DOI: 10.1111/j.1744-7909.2010.00959.x. View

17.

Simon L, Voisin M, Tatout C, Probst A . Structure and Function of Centromeric and Pericentromeric Heterochromatin in Arabidopsis thaliana. Front Plant Sci. 2015; 6:1049. PMC: 4663263. DOI: 10.3389/fpls.2015.01049. View

18.

Zhu Q, Ramm K, Shivakkumar R, Dennis E, Upadhyaya N . The ANTHER INDEHISCENCE1 gene encoding a single MYB domain protein is involved in anther development in rice. Plant Physiol. 2004; 135(3):1514-25. PMC: 519067. DOI: 10.1104/pp.104.041459. View

19.

Ott A, Yang Y, Bhattacharyya M, Horner H, Palmer R, Sandhu D . Molecular Mapping of D₁, D₂ and ms5 Revealed Linkage between the Cotyledon Color Locus D₂ and the Male-Sterile Locus ms5 in Soybean. Plants (Basel). 2016; 2(3):441-54. PMC: 4844383. DOI: 10.3390/plants2030441. View