Chitooligosaccharides Prevents the Development of Colitis-Associated Colorectal Cancer by Modulating the Intestinal Microbiota and Mycobiota

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Oct 18

PMID 31620100

Citations 37

Authors

Minna Wu

Jianmin Li

Yunying An

Puze Li

Wancheng Xiong

JinSong Li

Dong Yan

Mingyong Wang

Genshen Zhong

Affiliations

Soon will be listed here.

Abstract

Gut microbes play a crucial role in the development of colorectal cancer. Chitooligosaccharides (COS), are oligomer that are depolymerized from chitosan and possess a wide range of biological activities. In this study, the effects of COS on colorectal cancer (CRC) development were evaluated using azoxymethane and dextran sulfate sodium (AOM/DSS) induced mouse model of CRC (CACM). In the COS-treated CRC group (CMCOS), COS protected mice from CRC by decreasing the disease activity index, tumor incidences and multiplicity, and the mRNA levels of COX-2, IL-6, TNF-α, IL-1β, IL-10, and IKK-β mRNA in colonic epithelial cells. The results of a cage-exchanged experiment, in which mice from the CACMe and CMCOSe treatments exchanged cages every day to interact with microbes, showed that gut microbes play an important role in preventing CAC by COS. The abundances of fecal bacteria (total bacteria, , , and butyrate-producing bacteria) were detected by qPCR on the 0th, 1st, 3rd, 6th, 9th, and 10th weekends. Furthermore, microbiota and mycobiota were analyzed by high-throughput sequencing on an Illumina MiSeq PE300 system. COS protected mice from CRC by reversing the imbalance of bacteria and fungi, especially by reducing the abundance of , , and , and increasing the levels of , butyrate-producing bacteria and .

Citing Articles

Chitosan lactate improves repeated closed head injury-generated motor and neurological dysfunctions in mice by impacting microbiota gut-brain axis.

Bazaz M, Padhy H, Dandekar M Metab Brain Dis. 2025; 40(1):81.

PMID: 39751900 DOI: 10.1007/s11011-024-01517-2.

Advances in Chitosan-Based Smart Hydrogels for Colorectal Cancer Treatment.

Piotrowska U, Orzechowska K Pharmaceuticals (Basel). 2024; 17(10).

PMID: 39458901 PMC: 11510048. DOI: 10.3390/ph17101260.

Unveiling the overlooked fungi: the vital of gut fungi in inflammatory bowel disease and colorectal cancer.

Huang Y, Wang Y, Huang X, Yu X Gut Pathog. 2024; 16(1):59.

PMID: 39407244 PMC: 11481806. DOI: 10.1186/s13099-024-00651-7.

Mitotic Spindle Positioning (MISP) Facilitates Colorectal Cancer Progression by Forming a Complex with Opa Interacting Protein 5 (OIP5) and Activating the JAK2-STAT3 Signaling Pathway.

Hiura K, Watanabe M, Hirose N, Nakano K, Okamura T, Sasaki H Int J Mol Sci. 2024; 25(5).

PMID: 38474305 PMC: 10931608. DOI: 10.3390/ijms25053061.

Broccoli Cultivated with Deep Sea Water Mineral Fertilizer Enhances Anti-Cancer and Anti-Inflammatory Effects of AOM/DSS-Induced Colorectal Cancer in C57BL/6N Mice.

Lee Y, Pan Y, Lim D, Park S, Sin S, Kwack K Int J Mol Sci. 2024; 25(3).

PMID: 38338927 PMC: 10855752. DOI: 10.3390/ijms25031650.

References

Buffington S, Di Prisco G, Auchtung T, Ajami N, Petrosino J, Costa-Mattioli M . Microbial Reconstitution Reverses Maternal Diet-Induced Social and Synaptic Deficits in Offspring. Cell. 2016; 165(7):1762-1775. PMC: 5102250. DOI: 10.1016/j.cell.2016.06.001. View

Lang M, Berry D, Passecker K, Mesteri I, Bhuju S, Ebner F . HuR Small-Molecule Inhibitor Elicits Differential Effects in Adenomatosis Polyposis and Colorectal Carcinogenesis. Cancer Res. 2017; 77(9):2424-2438. PMC: 5826591. DOI: 10.1158/0008-5472.CAN-15-1726. View

Liguori G, Lamas B, Richard M, Brandi G, Da Costa G, Hoffmann T . Fungal Dysbiosis in Mucosa-associated Microbiota of Crohn's Disease Patients. J Crohns Colitis. 2015; 10(3):296-305. PMC: 4957473. DOI: 10.1093/ecco-jcc/jjv209. View

Liang T, Chen W, Lin Z, Kuo Y, Nguyen A, Pan P . An Amphiprotic Novel Chitosanase from Bacillus mycoides and Its Application in the Production of Chitooligomers with Their Antioxidant and Anti-Inflammatory Evaluation. Int J Mol Sci. 2016; 17(8). PMC: 5000699. DOI: 10.3390/ijms17081302. View

Tong C, Yin Z, Song Z, Dockendorff A, Huang C, Mariadason J . c-Jun NH2-terminal kinase 1 plays a critical role in intestinal homeostasis and tumor suppression. Am J Pathol. 2007; 171(1):297-303. PMC: 1941582. DOI: 10.2353/ajpath.2007.061036. View

Louis P, Hold G, Flint H . The gut microbiota, bacterial metabolites and colorectal cancer. Nat Rev Microbiol. 2014; 12(10):661-72. DOI: 10.1038/nrmicro3344. View

Snider A, Bialkowska A, Ghaleb A, Yang V, Obeid L, Hannun Y . Murine Model for Colitis-Associated Cancer of the Colon. Methods Mol Biol. 2016; 1438:245-54. PMC: 5657253. DOI: 10.1007/978-1-4939-3661-8_14. View

Higashimura Y, Naito Y, Takagi T, Uchiyama K, Mizushima K, Ushiroda C . Protective effect of agaro-oligosaccharides on gut dysbiosis and colon tumorigenesis in high-fat diet-fed mice. Am J Physiol Gastrointest Liver Physiol. 2016; 310(6):G367-75. DOI: 10.1152/ajpgi.00324.2015. View

Han F, Yang S, Lin M, Chen Y, Yang P, Xu J . Chitooligosaccharides promote radiosensitivity in colon cancer line SW480. World J Gastroenterol. 2016; 22(22):5193-200. PMC: 4893466. DOI: 10.3748/wjg.v22.i22.5193. View

10.

Sokol H, Leducq V, Aschard H, Pham H, Jegou S, Landman C . Fungal microbiota dysbiosis in IBD. Gut. 2016; 66(6):1039-1048. PMC: 5532459. DOI: 10.1136/gutjnl-2015-310746. View

11.

Plovier H, Everard A, Druart C, Depommier C, Van Hul M, Geurts L . A purified membrane protein from Akkermansia muciniphila or the pasteurized bacterium improves metabolism in obese and diabetic mice. Nat Med. 2016; 23(1):107-113. DOI: 10.1038/nm.4236. View

12.

. Structure, function and diversity of the healthy human microbiome. Nature. 2012; 486(7402):207-14. PMC: 3564958. DOI: 10.1038/nature11234. View

13.

Bhatt R, Kothari S, Gohil D, DSouza M, Chowdhary A . Novel evidence of microglial immune response in impairment of Dengue infection of CNS. Immunobiology. 2015; 220(10):1170-6. DOI: 10.1016/j.imbio.2015.06.002. View

14.

Chang P, Hao L, Offermanns S, Medzhitov R . The microbial metabolite butyrate regulates intestinal macrophage function via histone deacetylase inhibition. Proc Natl Acad Sci U S A. 2014; 111(6):2247-52. PMC: 3926023. DOI: 10.1073/pnas.1322269111. View

15.

Lewis J, Chen E, Baldassano R, Otley A, Griffiths A, Lee D . Inflammation, Antibiotics, and Diet as Environmental Stressors of the Gut Microbiome in Pediatric Crohn's Disease. Cell Host Microbe. 2015; 18(4):489-500. PMC: 4633303. DOI: 10.1016/j.chom.2015.09.008. View

16.

Zurlo D, Leone C, Assante G, Salzano S, Renzone G, Scaloni A . Cladosporol a stimulates G1-phase arrest of the cell cycle by up-regulation of p21(waf1/cip1) expression in human colon carcinoma HT-29 cells. Mol Carcinog. 2011; 52(1):1-17. DOI: 10.1002/mc.20872. View

17.

Dao M, Everard A, Aron-Wisnewsky J, Sokolovska N, Prifti E, Verger E . Akkermansia muciniphila and improved metabolic health during a dietary intervention in obesity: relationship with gut microbiome richness and ecology. Gut. 2015; 65(3):426-36. DOI: 10.1136/gutjnl-2014-308778. View

18.

Wu M, Wu Y, Deng B, Li J, Cao H, Qu Y . Isoliquiritigenin decreases the incidence of colitis-associated colorectal cancer by modulating the intestinal microbiota. Oncotarget. 2016; 7(51):85318-85331. PMC: 5356739. DOI: 10.18632/oncotarget.13347. View

19.

Van Raay T, Allen-Vercoe E . Microbial Interactions and Interventions in Colorectal Cancer. Microbiol Spectr. 2017; 5(3). PMC: 11687491. DOI: 10.1128/microbiolspec.BAD-0004-2016. View

20.

Mattaveewong T, Wongkrasant P, Chanchai S, Pichyangkura R, Chatsudthipong V, Muanprasat C . Chitosan oligosaccharide suppresses tumor progression in a mouse model of colitis-associated colorectal cancer through AMPK activation and suppression of NF-κB and mTOR signaling. Carbohydr Polym. 2016; 145:30-6. DOI: 10.1016/j.carbpol.2016.02.077. View