Keel Fracture Causes Stress and Inflammatory Responses and Inhibits the Expression of the Orexin System in Laying Hens

Overview

Journal Animals (Basel)

Date 2019 Oct 18

PMID 31618855

Citations 6

Authors

Haidong Wei

Chun Li

Hongwei Xin

Shuang Li

Yanju Bi

Xiang Li

Jianhong Li

Runxiang Zhang

Jun Bao

Affiliations

Soon will be listed here.

Abstract

Keel fracture has negative effects on the health and welfare of laying hens. We investigated effects of keel fracture on stress, inflammation, and the orexin system in laying hens. Ninety 17-week-old Lohmann white laying hens were palpated and euthanatized at 42 weeks old, and marked as normal keel (NK)/fractured keel (FK) from absence/presence of keel fracture. Serum, brain, liver, and abdominal-muscle samples were collected from 10 NK and 10 FK hens to determine the stress and inflammatory responses and the activity of orexin systems by corticosterone content, expression of heat shock proteins (, 70, 90), and inflammatory factors (tumor necrosis factor , nuclear factor-kappa Bp65 (), inducible nitric oxide synthase (), prostaglandin E synthases (), cyclooxygenase-2 (), interleukin-1β ()), orexin (), and orexin-receptor 1/2 (). The FK hens had higher serum corticosterone content, Hsps, and inflammatory factor mRNA expression levels than NK hens, although levels of in the liver and in the muscle were similar. Protein levels of Hsp70 and Hsp90 in the brain and liver, iNOS and COX-2 in the liver, NF-κBp65, iNOS, and COX-2 in the brain of FK hens were increased compared with NK hens. Furthermore, FK hens had lower mRNA expression of , , and than NK hens. Therefore, keel fracture causes stress and inflammation, and inhibits the expression of the orexin system in laying hens.

Citing Articles

Understanding the Causes of Keel Bone Damage and Its Effects on the Welfare of Laying Hens.

Li X, Cai X, Wang X, Zhu L, Yan H, Yao J Animals (Basel). 2025; 14(24.

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Avian Orexin: Feed Intake Regulator or Something Else?.

Ramser A, Dridi S Vet Sci. 2022; 9(3).

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Keel bone damage affects behavioral and physiological responses related to stress and fear in two strains of laying hens.

Wei H, Feng Y, Ding S, Nian H, Yu H, Zhao Q J Anim Sci. 2022; 100(4).

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Abnormal Bone Metabolism May Be a Primary Causative Factor of Keel Bone Fractures in Laying Hens.

Wei H, Chen Y, Nian H, Wang J, Liu Y, Wang J Animals (Basel). 2021; 11(11).

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Keel bone fractures in Danish laying hens: Prevalence and risk factors.

Thofner I, Dahl J, Christensen J PLoS One. 2021; 16(8):e0256105.

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