Neuroprotective Effects and Mechanisms of Zhenlong Xingnao Capsule in and Models of Hypoxia

Overview

Journal Front Pharmacol

Date 2019 Oct 16

PMID 31611791

Citations 2

Authors

Xia Wei

Qingfen Zhu

Na Liu

LiHua Xu

Sheng Wei

Zhiyun Fan

Changhua Sun

Yan Zhao

Mingqi Qiao

Jibiao Wu

Defu Hu

Yang Wang

Peng Sun

Affiliations

Soon will be listed here.

Abstract

Zhenlong Xingnao Capsule (ZXC) is a Tibetan medicine used to treat ischemic stroke. In this study, we determined the and effects of ZXC on reactive oxygen species (ROS) in a mouse BV-2 microglial cell hypoxia-reoxygenation and rat middle cerebral artery occlusion infarction models. We aimed to clarify the role of ZXC in cerebral ischemia protection; reveal amino acid neurotransmitter changes in the frontal cortex after drug intervention; determine mRNA and protein expression changes in Bcl-2, Bax, caspase-3, P38, and nuclear factor (NF)-кB in the frontal cortex and changes in antioxidant indices in the brain; and elucidate the mechanisms underlying ZXC action. After hypoxia-reoxygenation, ROS levels were significantly increased in BV-2 cells, and their levels decreased after treatment with ZXC. ZXC had protective effects on ischemic/anoxic injury and by downregulating the expressions of caspase-3 and NF-кB mRNA during ischemia and reperfusion and that of p38 and caspase-3 during acute ischemia and reperfusion as well as the steady-state levels of excitatory amino acids/inhibitory amino acids and by improving the total antioxidant capacity and total superoxide dismutase activities during ischemia. These findings provide new molecular evidence for the mechanisms underlying ZXC action.

Citing Articles

Mfn2 Overexpression Attenuates Cardio-Cerebrovascular Ischemia-Reperfusion Injury Through Mitochondrial Fusion and Activation of the AMPK/Sirt3 Signaling.

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PMID: 33195281 PMC: 7644524. DOI: 10.3389/fcell.2020.598078.

Mitochondrial MPTP: A Novel Target of Ethnomedicine for Stroke Treatment by Apoptosis Inhibition.

Li Y, Sun J, Wu R, Bai J, Hou Y, Zeng Y Front Pharmacol. 2020; 11:352.

PMID: 32269527 PMC: 7109312. DOI: 10.3389/fphar.2020.00352.

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