Temporal and Spatial Expression of Arabidopsis Gene Homologs Control Daylength Adaptation and Bulb Formation in Onion (Allium Cepa L.)

Overview

Journal Sci Rep

Specialty Science

Date 2019 Oct 12

PMID 31601963

Citations 8

Authors

Md Harun Ar Rashid

Wei Cheng

Brian Thomas

Affiliations

Soon will be listed here.

Abstract

Genetic studies aimed at onion improvement have been limited because of high heterozygosity, a very large genome size with a high level of repetitive DNA and a biennial life cycle. Onion bulb initiation is daylength-dependent, which places a significant barrier to adapting new varieties for growth at different latitudes. Compared to the photoperiodic regulation of flowering, relatively little is known about genetic regulation of the bulbing process. This study aims to identify the role of gene sequences involved in daylength-regulated bulb formation and tissue specific expression of onion. A comprehensive set of developmental and spatial quantitative mRNA expression experiments were carried out to investigate expression of onion FLOWERING LOCUS T (AcFT), LEAFY (AcLFY) and GIBBERELLIN-3 OXIDASE (GA3ox1) during the bulbing response. Bulbing ratios were used to measure the response of onion plants under long day (LD) and short day (SD) conditions. AcFT1 was expressed in LD, which induces bulb formation, while AcFT4 was expressed in SD, which inhibits bulb formation. AcFT5 and AcFT6 were expressed in LD and might also be involved in bulb formation itself. All AcFT, AcLFY and GA3ox1 genes showed distinctive patterns of tissue specific expression in onion, with AcFT genes found primarily in the sites of perception in the leaf and LFY in the basal tissues, the site of response. The results are consistent with AcFT1 expression being the signal for LD-induced bulb initiation and AcFT4, being involved in suppressing bulbing in SD.

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References

Osterlund M, Paterson A . Applied plant genomics: the secret is integration. Curr Opin Plant Biol. 2002; 5(2):141-5. DOI: 10.1016/s1369-5266(02)00246-7. View

Taylor A, Massiah A, Thomas B . Conservation of Arabidopsis thaliana photoperiodic flowering time genes in onion (Allium cepa L.). Plant Cell Physiol. 2010; 51(10):1638-47. DOI: 10.1093/pcp/pcq120. View

Blazquez , Green , Nilsson , Sussman , WEIGEL . Gibberellins promote flowering of arabidopsis by activating the LEAFY promoter . Plant Cell. 1998; 10(5):791-800. PMC: 144373. DOI: 10.1105/tpc.10.5.791. View

Laurie R, Diwadkar P, Jaudal M, Zhang L, Hecht V, Wen J . The Medicago FLOWERING LOCUS T homolog, MtFTa1, is a key regulator of flowering time. Plant Physiol. 2011; 156(4):2207-24. PMC: 3149922. DOI: 10.1104/pp.111.180182. View

Jaeger K, Wigge P . FT protein acts as a long-range signal in Arabidopsis. Curr Biol. 2007; 17(12):1050-4. DOI: 10.1016/j.cub.2007.05.008. View

Lee R, Baldwin S, Kenel F, McCallum J, Macknight R . FLOWERING LOCUS T genes control onion bulb formation and flowering. Nat Commun. 2013; 4:2884. DOI: 10.1038/ncomms3884. View

Purwestri Y, Ogaki Y, Tamaki S, Tsuji H, Shimamoto K . The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a. Plant Cell Physiol. 2009; 50(3):429-38. DOI: 10.1093/pcp/pcp012. View

Pin P, Nilsson O . The multifaceted roles of FLOWERING LOCUS T in plant development. Plant Cell Environ. 2012; 35(10):1742-55. DOI: 10.1111/j.1365-3040.2012.02558.x. View

Wang L, Liang H, Pang J, Zhu M . [Regulation network and biological roles of LEAFY in Arabidopsis thaliana in floral development]. Yi Chuan. 2005; 26(1):137-42. View

10.

McCallum J, Baldwin S, Shigyo M, Deng Y, van Heusden S, Pither-Joyce M . AlliumMap-A comparative genomics resource for cultivated Allium vegetables. BMC Genomics. 2012; 13:168. PMC: 3423043. DOI: 10.1186/1471-2164-13-168. View

11.

Hao X, Chao W, Yang Y, Horvath D . Coordinated Expression of FLOWERING LOCUS T and DORMANCY ASSOCIATED MADS-BOX-Like Genes in Leafy Spurge. PLoS One. 2015; 10(5):e0126030. PMC: 4427404. DOI: 10.1371/journal.pone.0126030. View

12.

Bohlenius H, Huang T, Charbonnel-Campaa L, Brunner A, Jansson S, Strauss S . CO/FT regulatory module controls timing of flowering and seasonal growth cessation in trees. Science. 2006; 312(5776):1040-3. DOI: 10.1126/science.1126038. View

13.

Navarro C, Abelenda J, Cruz-Oro E, Cuellar C, Tamaki S, Silva J . Control of flowering and storage organ formation in potato by FLOWERING LOCUS T. Nature. 2011; 478(7367):119-22. DOI: 10.1038/nature10431. View

14.

Siriwardana N, Lamb R . The poetry of reproduction: the role of LEAFY in Arabidopsis thaliana flower formation. Int J Dev Biol. 2012; 56(4):207-21. DOI: 10.1387/ijdb.113450ns. View

15.

Yoo S, Chung K, Kim J, Lee J, Hong S, Yoo S . CONSTANS activates SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 through FLOWERING LOCUS T to promote flowering in Arabidopsis. Plant Physiol. 2005; 139(2):770-8. PMC: 1255994. DOI: 10.1104/pp.105.066928. View

16.

Weigel D, Alvarez J, Smyth D, Yanofsky M, Meyerowitz E . LEAFY controls floral meristem identity in Arabidopsis. Cell. 1992; 69(5):843-59. DOI: 10.1016/0092-8674(92)90295-n. View

17.

Kardailsky I, Shukla V, Ahn J, Dagenais N, Christensen S, Nguyen J . Activation tagging of the floral inducer FT. Science. 1999; 286(5446):1962-5. DOI: 10.1126/science.286.5446.1962. View

18.

Andres F, Coupland G . The genetic basis of flowering responses to seasonal cues. Nat Rev Genet. 2012; 13(9):627-39. DOI: 10.1038/nrg3291. View

19.

Danilevskaya O, Meng X, McGonigle B, Muszynski M . Beyond flowering time: pleiotropic function of the maize flowering hormone florigen. Plant Signal Behav. 2011; 6(9):1267-70. PMC: 3258048. DOI: 10.4161/psb.6.9.16423. View

20.

Hsu C, Liu Y, Luthe D, Yuceer C . Poplar FT2 shortens the juvenile phase and promotes seasonal flowering. Plant Cell. 2006; 18(8):1846-61. PMC: 1533980. DOI: 10.1105/tpc.106.041038. View