Long Noncoding RNA CASC15 is Upregulated in Non-small Cell Lung Cancer and Facilitates Cell Proliferation and Metastasis Via Targeting MiR-130b-3p

Objective: Recent researches have discovered a class of long noncoding RNAs (lncRNAs), which are dysregulated in various tumors and linked to carcinogenesis. This study aims to uncover the molecular functions of lncRNA CASC15 in non-small cell lung cancer (NSCLC) tumorigenesis.

Patients And Methods: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was performed to detect CASC15 expression in 55 NSCLC samples and four NSCLC cell lines. Besides, the function of CASC15 was detected through proliferation assay, transwell assay, and wound healing assay in NSCLC cells. Furthermore, the interaction between CASC15 and miR-130b-3p in NSCLC was studied by performing dual-luciferase reporter assay. In addition, tumor formation and metastasis assay were performed in vivo.

Results: CASC15 expression was remarkably upregulated in NSCLC samples compared with that in adjacent samples. Cell proliferation, invasion, and migration in NSCLC were inhibited via knockdown of CASC15 in vitro. Moreover, RT-qPCR results revealed that miR-130b-3p was upregulated via knockdown of CASC15 in vitro. In addition, miR-130b-3p was a direct target of CASC15 in NSCLC. Tumor formation and metastasis were inhibited after CASC15 was knockdown in vivo.

Conclusions: Our study indicates that CASC15 could promote metastasis and proliferation of NSCLC through sponging miR-130b-3p in vitro and in vivo, which may offer a new therapeutic intervention for NSCLC patients.