Modular Organization of Cerebellar Climbing Fiber Inputs During Goal-directed Behavior

Overview

Journal Elife

Specialty Biology

Date 2019 Oct 10

PMID 31596238

Citations 18

Authors

Shinichiro Tsutsumi

Naoki Hidaka

Yoshikazu Isomura

Masanori Matsuzaki

Kenji Sakimura

Masanobu Kano

Kazuo Kitamura

Affiliations

Soon will be listed here.

Abstract

The cerebellum has a parasagittal modular architecture characterized by precisely organized climbing fiber (CF) projections that are congruent with alternating aldolase C/zebrin II expression. However, the behavioral relevance of CF inputs into individual modules remains poorly understood. Here, we used two-photon calcium imaging in the cerebellar hemisphere Crus II in mice performing an auditory go/no-go task to investigate the functional differences in CF inputs to modules. CF signals in medial modules show anticipatory decreases, early increases, secondary increases, and reward-related increases or decreases, which represent quick motor initiation, go cues, fast motor behavior, and positive reward outcomes. CF signals in lateral modules show early increases and reward-related decreases, which represent no-go and/or go cues and positive reward outcomes. The boundaries of CF functions broadly correspond to those of aldolase C patterning. These results indicate that spatially segregated CF inputs in different modules play distinct roles in the execution of goal-directed behavior.

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