Detection of 8-Hydroxy-2'-Deoxyguanosine Biomarker with a Screen-Printed Electrode Modified with Graphene

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2019 Oct 9

PMID 31590208

Citations 3

Authors

Codruta Varodi

Florina Pogacean

Maria Coros

Marcela-Corina Rosu

Raluca-Ioana Stefan-van Staden

Emese Gal

Lucian-Barbu Tudoran

Stela Pruneanu

Simona Mirel

Affiliations

Soon will be listed here.

Abstract

In this work we present the preparation of graphene material by exfoliation of graphite rods via pulses of current in electrolyte, containing a mixture of boric acid (0.05 M) and sodium chloride (0.05 M). The material was morphologically and structurally characterized by SEM/TEM/HR-TEM, XRD and FTIR techniques. TEM investigation of graphene flakes deposited onto carbon-coated grids allowed the visualization of thin and transparent regions, attributed to few-layer graphene (FLG), as well as thick and dark regions attributed to multi-layer graphene (MLG). The mixed composition of the material was additionally confirmed by XRD, which further indicated that the amount of FLG within the sample was around 83%, while MLG was around 17%. The performance of a screen-printed electrode (SPE) modified with graphene (SPE-Gr) was tested for 8-hydroxy-2'-deoxyguanosine detection. The graphene-modified electrode had a higher sensitivity in comparison with that of SPE, both in standard laboratory solutions (phosphate buffered saline-PBS) and in human saliva.

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