A CII-dependent Promoter is Located Within the Q Gene of Bacteriophage Lambda

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 May 1

PMID 3159014

Citations 24

Authors

B C Hoopes

W R McClure

Affiliations

Soon will be listed here.

Abstract

We have found a cII-dependent promoter, PaQ, within the Q gene of bacteriophage lambda. Transcription experiments and abortive initiation assays performed in vitro showed that the promoter strength and the cII affinity of PaQ were comparable to the other cII-dependent lambda promoters, PE and PI. The location and leftward direction of PaQ suggests a possible role in the delay of lambda late-gene expression by cII protein, a phenomenon that has been called cII-dependent inhibition. We have constructed a promoter down mutation, paq-1, by changing a single base pair in the putative cII binding site of the promoter by oligonucleotide site-directed mutagenesis. The paq-1 mutant promoter required about 4-fold higher cII concentrations for maximal activation compared to the wild-type PaQ. We tested the hypothesis that PaQ is responsible in part for the delay of lambda late-gene expression by recombining the paq-1 mutation into a phage showing severe cII-dependent inhibition. We found that the paq-1 mutation relieved the cII-dependent growth defect of this phage. The paq-1 mutation (in combination with lambda cI857) resulted in a clear-plaque phenotype at the permissive temperature of 32 degrees C. The role of the PaQ-initiated antisense transcript in the control of lambda development is discussed.

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References

Birnboim H, DOLY J . A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6):1513-23. PMC: 342324. DOI: 10.1093/nar/7.6.1513. View

Ho Y, Lewis M, Rosenberg M . Purification and properties of a transcriptional activator. The cII protein of phage lambda. J Biol Chem. 1982; 257(15):9128-34. View

Cech C, McClure W . Characterization of ribonucleic acid polymerase-T7 promoter binary complexes. Biochemistry. 1980; 19(11):2440-7. DOI: 10.1021/bi00552a023. View

Schmeissner U, Court D, Shimatake H, Rosenberg M . Promoter for the establishment of repressor synthesis in bacteriophage lambda. Proc Natl Acad Sci U S A. 1980; 77(6):3191-5. PMC: 349580. DOI: 10.1073/pnas.77.6.3191. View

Herskowitz I, Hagen D . The lysis-lysogeny decision of phage lambda: explicit programming and responsiveness. Annu Rev Genet. 1980; 14:399-445. DOI: 10.1146/annurev.ge.14.120180.002151. View

Simatake H, Rosenberg M . Purified lambda regulatory protein cII positively activates promoters for lysogenic development. Nature. 1981; 292(5819):128-32. DOI: 10.1038/292128a0. View

Oppenheim A, Gottesman S, Gottesman M . Regulation of bacteriophage lambda int gene expression. J Mol Biol. 1982; 158(3):327-46. DOI: 10.1016/0022-2836(82)90201-7. View

Enea V, Zinder N . Interference resistant mutants of phage f1. Virology. 1982; 122(1):222-6. DOI: 10.1016/0042-6822(82)90395-6. View

Joyce C, Grindley N . Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli. Proc Natl Acad Sci U S A. 1983; 80(7):1830-4. PMC: 393703. DOI: 10.1073/pnas.80.7.1830. View

10.

HAWLEY D, McClure W . Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983; 11(8):2237-55. PMC: 325881. DOI: 10.1093/nar/11.8.2237. View

11.

Ho Y, WULFF D, Rosenberg M . Bacteriophage lambda protein cII binds promoters on the opposite face of the DNA helix from RNA polymerase. Nature. 1983; 304(5928):703-8. DOI: 10.1038/304703a0. View

12.

Simons R, Kleckner N . Translational control of IS10 transposition. Cell. 1983; 34(2):683-91. DOI: 10.1016/0092-8674(83)90401-4. View

13.

Shih M, Gussin G . Differential effects of mutations on discrete steps in transcription initiation at the lambda PRE promoter. Cell. 1983; 34(3):941-9. DOI: 10.1016/0092-8674(83)90551-2. View

14.

Amann E, Brosius J, Ptashne M . Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene. 1983; 25(2-3):167-78. DOI: 10.1016/0378-1119(83)90222-6. View

15.

Shih M, Gussin G . Role of cII protein in stimulating transcription initiation at the lambda PRE promoter. Enhanced formation and stabilization of open complexes. J Mol Biol. 1984; 172(4):489-506. DOI: 10.1016/s0022-2836(84)80019-4. View

16.

Mizuno T, Chou M, Inouye M . A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA). Proc Natl Acad Sci U S A. 1984; 81(7):1966-70. PMC: 345417. DOI: 10.1073/pnas.81.7.1966. View

17.

Coleman J, Green P, Inouye M . The use of RNAs complementary to specific mRNAs to regulate the expression of individual bacterial genes. Cell. 1984; 37(2):429-36. DOI: 10.1016/0092-8674(84)90373-8. View

18.

Zagursky R, Berman M . Cloning vectors that yield high levels of single-stranded DNA for rapid DNA sequencing. Gene. 1984; 27(2):183-91. DOI: 10.1016/0378-1119(84)90139-2. View

19.

Shih M, Gussin G . Kinetic analysis of mutations affecting the cII activation site at the PRE promoter of bacteriophage lambda. Proc Natl Acad Sci U S A. 1984; 81(20):6432-6. PMC: 391938. DOI: 10.1073/pnas.81.20.6432. View

20.

Butler B, Echols H . Regulation of bacteriophage lambda development by gene N: properties of a mutation that bypasses N control of late protein synthesis. Virology. 1970; 40(2):212-22. DOI: 10.1016/0042-6822(70)90396-x. View