Physical Mapping of Stem Rust Resistance Gene Sr52 from Based on -Induced Homoeologous Recombination

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 Oct 5

PMID 31581639

Citations 8

Authors

Huanhuan Li

Zhenjie Dong

Chao Ma

Xiubin Tian

Zengjun Qi

Nan Wu

Bernd Friebe

Zhiguo Xiang

Qing Xia

Wenxuan Liu

Tianya Li

Affiliations

Soon will be listed here.

Abstract

Wheat stem rust caused by f. sp. () had been a devastating foliar disease worldwide during the 20th century. With the emergence of Ug99 races, which are virulent to most stem rust resistance genes deployed in wheat varieties and advanced lines, stem rust has once again become a disease threatening global wheat production. , derived from and mapped to the long arm of 6V#3, is one of the few effective genes against Ug99 races. In this study, the wheat-. Robertsonian translocation T6AS·6V#3L, the only stock carrying released to experimental and breeding programs so far, was crossed with a CS mutant to induce recombinants with shortened 6V#3L chromosome segments locating . Six independent homozygous recombinants with different segment sizes and breakpoints were developed and characterized using hybridization and molecular markers analyses. Stem rust resistance evaluation showed that only three terminal recombinants (1381, 1380, and 1392) containing 8%, 22%, and 30% of the distal segment of 6V#3L, respectively, were resistant to stem rust. Thus, the gene was mapped into 6V#3L bin FL 0.92-1.00. In addition, three molecular markers in the -located interval of 6V#3L were confirmed to be diagnostic markers for selection of introgressed into common wheat. The newly developed small segment translocation lines with Sr52 and the identified molecular markers closely linked to Sr52 will be valuable for wheat disease breeding.

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