Transposon Mutagenesis Reveals Pv. Optimizes Its Virulence Factors for Pathogenicity on Different Hosts

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2019 Oct 4

PMID 31579596

Citations 9

Authors

Nanami Sakata

Takako Ishiga

Haruka Saito

Viet Tru Nguyen

Yasuhiro Ishiga

Affiliations

Soon will be listed here.

Abstract

pv. (), which causes bacterial blight disease of Brassicaceae, is an economically important pathogen worldwide. To identify genes involved in pathogenesis, we conducted a screen for 1,040 individual KB211 Tn mutants with reduced virulence on cabbage plants using a dip-inoculation method. We isolated 53 reduced virulence mutants and identified several potential virulence factors involved in virulence mechanisms such as the type III secretion system, membrane transporters, transcription factors, and amino acid metabolism. Importantly, is pathogenic on a range of monocotyledonous and dicotyledonous plants. Therefore, we also carried out the inoculation test on oat plants, which are cultivated after cabbage cultivation as green manure crops. Interestingly among the 53 mutants, 31 mutants also exhibited reduced virulence on oat seedlings, indicating that optimizes its virulence factors for pathogenicity on different host plants. Our results highlight the importance of revealing the virulence factors for each plant host-bacterial interaction, and will provide new insights into virulence mechanisms.

Citing Articles

HexR Transcription Factor Contributes to pv. Virulence by Coordinating Type Three Secretion System Genes.

Sakata N, Fujikawa T, Uke A, Ishiga T, Ichinose Y, Ishiga Y Microorganisms. 2023; 11(4).

PMID: 37110448 PMC: 10145369. DOI: 10.3390/microorganisms11041025.

Prevention of Stomatal Entry as a Strategy for Plant Disease Control against Foliar Pathogenic Species.

Sakata N, Ishiga Y Plants (Basel). 2023; 12(3).

PMID: 36771673 PMC: 9919041. DOI: 10.3390/plants12030590.

Large-Scale Transposon Mutagenesis Reveals Type III Secretion Effector HopR1 Is a Major Virulence Factor in pv. .

Ishiga T, Sakata N, Usuki G, Nguyen V, Gomi K, Ishiga Y Plants (Basel). 2023; 12(1).

PMID: 36616271 PMC: 9823363. DOI: 10.3390/plants12010141.

pv. Virulence Factors Are Involved in Resistance to Plant-Derived Antimicrobials during Infection.

Sakata N, Haraguchi T, Masuo S, Ishiga T, Ishiga Y Plants (Basel). 2022; 11(13).

PMID: 35807692 PMC: 9269351. DOI: 10.3390/plants11131742.

Isolation and Complete Genome Sequence Analysis of Pa82, a Novel Pathogen Causing Bacterial Wilt on Patchouli.

Zhang Y, Wang B, Li Q, Huang D, Zhang Y, Li G Front Microbiol. 2022; 12:818228.

PMID: 35095821 PMC: 8795763. DOI: 10.3389/fmicb.2021.818228.

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