Filamentation Initiated by Cas2 and Its Association with the Acquisition Process in Cells

Overview

Journal Int J Oral Sci

Date 2019 Oct 4

PMID 31578319

Citations 4

Authors

Lei Wang

Xin Yu

Mengjie Li

Guiqin Sun

Lin Zou

Tiansheng Li

Linlin Hou

Yameng Guo

Danfeng Shen

Di Qu

Xunjia Cheng

Li Chen

Affiliations

Soon will be listed here.

Abstract

Cas1-and-Cas2-mediated new spacer acquisition is an essential process for bacterial adaptive immunity. The process is critical for the ecology of the oral microflora and oral health. Although molecular mechanisms for spacer acquisition are known, it has never been established if this process is associated with the morphological changes of bacteria. In this study, we demonstrated a novel Cas2-induced filamentation phenotype in E. coli that was regulated by co-expression of the Cas1 protein. A 30 amino acid motif at the carboxyl terminus of Cas2 is necessary for this function. By imaging analysis, we provided evidence to argue that Cas-induced filamentation is a step coupled with new spacer acquisition during which filaments are characterised by polyploidy with asymmetric cell division. This work may open new opportunities to investigate the adaptive immune response and microbial balance for oral health.

Citing Articles

Structural and functional characterization of Cas2 of CRISPR-Cas subtype I-C lacking the CRISPR component.

Anand V, Sheeja Prabhakaran H, Gogoi P, Kanaujia S, Kumar M Front Mol Biosci. 2022; 9:988569.

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Filamentous morphology of bacterial pathogens: regulatory factors and control strategies.

Khan F, Jeong G, Tabassum N, Mishra A, Kim Y Appl Microbiol Biotechnol. 2022; 106(18):5835-5862.

PMID: 35989330 DOI: 10.1007/s00253-022-12128-1.

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Yilmaz S EXCLI J. 2021; 20:19-45.

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Growth inhibition by bacterial Cas2Em proteins expressed in mammalian cells.

Yu X, Wang L, Zou L, Li M, Li T, Hou L Am J Transl Res. 2020; 12(6):2499-2520.

PMID: 32655787 PMC: 7344093.

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