Micropatterned Nanolayers Immobilized with Nerve Growth Factor for Neurite Formation of PC12 Cells

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2019 Oct 2

PMID 31571871

Citations 6

Authors

Seong Min Kim

Masashi Ueki

Xueli Ren

Jun Akimoto

Yasuyuki Sakai

Yoshihiro Ito

Affiliations

Soon will be listed here.

Abstract

Background: Nerve regeneration is important for the treatment of degenerative diseases and neurons injured by accidents. Nerve growth factor (NGF) has been previously conjugated to materials for promotion of neurogenesis.

Materials And Methods: Photoreactive gelatin was prepared by chemical coupling of gelatin with azidobenzoic acid (P-gel), and then NGF was immobilized on substrates in the presence or absence of micropatterned photomasks. UV irradiation induced crosslinking reactions of P-gel with itself, NGF, and the plate for immobilization.

Results: By adjustment of the P-gel concentration, the nanometer-order height of micropatterns was controlled. NGF was quantitatively immobilized with increasing amounts of P-gel. Immobilized NGF induced neurite outgrowth of PC12 cells, a cell line derived from a pheochromocytoma of the rat adrenal medulla, at the same level as soluble NGF. The immobilized NGF showed higher thermal stability than the soluble NGF and was repeatedly used without loss of biological activity. The 3D structure (height of the formed micropattern) regulated the behavior of neurite guidance. As a result, the orientation of neurites was regulated by the stripe pattern width.

Conclusion: The micropattern-immobilized NGF nanolayer biochemically and topologically regulated neurite formation.

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References

Ito Y . Surface micropatterning to regulate cell functions. Biomaterials. 1999; 20(23-24):2333-42. DOI: 10.1016/s0142-9612(99)00162-3. View

Piper M, Salih S, Weinl C, Holt C, Harris W . Endocytosis-dependent desensitization and protein synthesis-dependent resensitization in retinal growth cone adaptation. Nat Neurosci. 2005; 8(2):179-86. PMC: 3682638. DOI: 10.1038/nn1380. View

Gundersen R, Barrett J . Neuronal chemotaxis: chick dorsal-root axons turn toward high concentrations of nerve growth factor. Science. 1979; 206(4422):1079-80. DOI: 10.1126/science.493992. View

Gomez N, Schmidt C . Nerve growth factor-immobilized polypyrrole: bioactive electrically conducting polymer for enhanced neurite extension. J Biomed Mater Res A. 2006; 81(1):135-49. PMC: 2917345. DOI: 10.1002/jbm.a.31047. View

Zeng J, Huang Z, Yin G, Qin J, Chen X, Gu J . Fabrication of conductive NGF-conjugated polypyrrole-poly(l-lactic acid) fibers and their effect on neurite outgrowth. Colloids Surf B Biointerfaces. 2013; 110:450-7. DOI: 10.1016/j.colsurfb.2013.05.012. View

Chua J, Chng C, Moe A, Tann J, Goh E, Chiam K . Extending neurites sense the depth of the underlying topography during neuronal differentiation and contact guidance. Biomaterials. 2014; 35(27):7750-61. DOI: 10.1016/j.biomaterials.2014.06.008. View

Joddar B, Guy A, Kamiguchi H, Ito Y . Spatial gradients of chemotropic factors from immobilized patterns to guide axonal growth and regeneration. Biomaterials. 2013; 34(37):9593-601. DOI: 10.1016/j.biomaterials.2013.08.019. View

Pinho A, Fonseca A, Serra A, Santos J, Coelho J . Peripheral Nerve Regeneration: Current Status and New Strategies Using Polymeric Materials. Adv Healthc Mater. 2016; 5(21):2732-2744. DOI: 10.1002/adhm.201600236. View

DiCosimo R, McAuliffe J, Poulose A, Bohlmann G . Industrial use of immobilized enzymes. Chem Soc Rev. 2013; 42(15):6437-74. DOI: 10.1039/c3cs35506c. View

10.

Haq F, Anandan V, Keith C, Zhang G . Neurite development in PC12 cells cultured on nanopillars and nanopores with sizes comparable with filopodia. Int J Nanomedicine. 2007; 2(1):107-15. PMC: 2673821. DOI: 10.2147/nano.2007.2.1.107. View

11.

Cao X, Shoichet M . Defining the concentration gradient of nerve growth factor for guided neurite outgrowth. Neuroscience. 2001; 103(3):831-40. DOI: 10.1016/s0306-4522(01)00029-x. View

12.

Sharma A, Zbarska S, Petersen E, Marti M, Mallapragada S, Sakaguchi D . Oriented growth and transdifferentiation of mesenchymal stem cells towards a Schwann cell fate on micropatterned substrates. J Biosci Bioeng. 2015; 121(3):325-35. DOI: 10.1016/j.jbiosc.2015.07.006. View

13.

Mateo C, Grazu V, Palomo J, Lopez-Gallego F, Fernandez-Lafuente R, Guisan J . Immobilization of enzymes on heterofunctional epoxy supports. Nat Protoc. 2007; 2(5):1022-33. DOI: 10.1038/nprot.2007.133. View

14.

Kitajima T, Obuse S, Adachi T, Tomita M, Ito Y . Recombinant human gelatin substitute with photoreactive properties for cell culture and tissue engineering. Biotechnol Bioeng. 2011; 108(10):2468-76. DOI: 10.1002/bit.23192. View

15.

Simitzi C, Karali K, Ranella A, Stratakis E . Controlling the Outgrowth and Functions of Neural Stem Cells: The Effect of Surface Topography. Chemphyschem. 2018; 19(10):1143-1163. DOI: 10.1002/cphc.201701175. View

16.

Ming G, Wong S, Henley J, Yuan X, Song H, Spitzer N . Adaptation in the chemotactic guidance of nerve growth cones. Nature. 2002; 417(6887):411-8. DOI: 10.1038/nature745. View

17.

Alhosseini S, Moztarzadeh F, Mozafari M, Asgari S, Dodel M, Samadikuchaksaraei A . Synthesis and characterization of electrospun polyvinyl alcohol nanofibrous scaffolds modified by blending with chitosan for neural tissue engineering. Int J Nanomedicine. 2012; 7:25-34. PMC: 3260948. DOI: 10.2147/IJN.S25376. View

18.

Haas A, Prigent S, Dutertre S, Le Drean Y, Le Page Y . Neurite analyzer: An original Fiji plugin for quantification of neuritogenesis in two-dimensional images. J Neurosci Methods. 2016; 271:86-91. DOI: 10.1016/j.jneumeth.2016.07.011. View

19.

Kapur T, Shoichet M . Immobilized concentration gradients of nerve growth factor guide neurite outgrowth. J Biomed Mater Res A. 2004; 68(2):235-43. DOI: 10.1002/jbm.a.10168. View

20.

Marquardt L, Sakiyama-Elbert S . Engineering peripheral nerve repair. Curr Opin Biotechnol. 2013; 24(5):887-92. PMC: 3783565. DOI: 10.1016/j.copbio.2013.05.006. View