Protective Efficacy of a Vaccine Using a -based Delivery System Expressing Omp3b, BCSP31, and SOD Proteins Against Brucellosis in Korean Black Goats

Overview

Journal Can J Vet Res

Specialty Veterinary Medicine

Date 2019 Oct 2

PMID 31571725

Citations 1

Authors

Won-Kyong Kim

Ja-Young Moon

Jeong-Sang Cho

Jin Hur

Affiliations

Soon will be listed here.

Abstract

This study aimed to assess the protective efficacy of a novel vaccine formulation in goats. Twenty black goats were separated into 2 groups. Group A was injected with 3.0 × 10 CFU (colony-forming units)/mL of a -based delivery system harboring only vector (pMMP65). Group B was immunized with 3.0 × 10 CFU/mL of the vaccine, a mixture of 3 vaccine strains (using a -based delivery system) expressing each recombinant Omp3b, BCSP31, and SOD protein. No delivery strain was isolated from all tested lymph nodes and parenchymal organs. Serum immunoglobulin (Ig) G titers and interferon gamma concentrations were significantly higher in group B than those in group A. After intraconjunctival challenge with virulent strain 544, 40% of the vaccinated animals in group B were protected against infection. The infection index and colonization of in tested tissues was significantly lower in group B than group A. We conclude that this vaccine induces significant antigen-specific immune responses and provides effective protection against infection in goats. Further studies are needed to enhance the protection rate of this vaccine and to discover its practical application in small ruminants.

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