RNase G Controls TpiA MRNA Abundance in Response to Oxygen Availability in Escherichia Coli

Overview

Journal J Microbiol

Specialty Microbiology

Date 2019 Oct 2

PMID 31571126

Citations 8

Authors

Jaejin Lee

Dong-Ho Lee

Che Ok Jeon

Kangseok Lee

Affiliations

Soon will be listed here.

Abstract

Studies have shown that many enzymes involved in glycolysis are upregulated in Escherichia coli endoribonuclease G (rng) null mutants. However, the molecular mechanisms underlying the RNase G-associated regulation of glycolysis have not been characterized. Here, we show that RNase G cleaves the 5' untranslated region of triosephosphate isomerase A (tpiA) mRNA, leading to destabilization of the mRNA in E. coli. Nucleotide substitutions within the RNase G cleavage site in the genome resulted in altered tpiA mRNA stability, indicating that RNase G activity influences tpiA mRNA abundance. In addition, we observed that tpiA expression was enhanced, whereas that of RNase G was decreased, in E. coli cells grown anaerobically. Our findings suggest that RNase G negatively regulates tpiA mRNA abundance in response to oxygen availability in E. coli.

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