Molecular Identification and Chromosomal Localization of New Powdery Mildew Resistance Gene Pm11 in Oat

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2019 Oct 2

PMID 31570968

Citations 6

Authors

Tomasz Ociepa

Sylwia Okon

Aleksandra Nucia

Justyna Lesniowska-Nowak

Edyta Paczos-Grzeda

Maciej Bisaga

Affiliations

Soon will be listed here.

Abstract

The appropriate selection of various traits in valuable plants is very important for modern plant breeding. Effective resistance to fungal diseases, such as powdery mildew, is an example of such a trait in oats. Marker-assisted selection is an important tool that reduces the time and cost of selection. The aims of the present study were the identification of dominant DArTseq markers associated with a new resistance gene, annotated as Pm11 and derived from Avena sterilis genotype CN113536, and the subsequent conversion of these markers into a PCR-based assay. Among the obtained 30,620 silicoDArT markers, 202 markers were highly associated with resistance in the analysed population. Of these, 71 were selected for potential conversion: 42 specific to resistant and 29 to susceptible individuals. Finally, 40 silicoDArT markers were suitable for primer design. From this pool, five markers, 3 for resistant and 2 for susceptible plants, were selected for product amplification in the expected groups. The developed method, based on 2 selection markers, provides certain identification of resistant and susceptible homozygotes. Also, the use of these markers allowed the determination of heterozygotes in the analysed population. Selected silicoDArT markers were also used for chromosomal localization of new resistance genes. Five out of 71 segregating silicoDArT markers for the Pm11 gene were found on the available consensus genetic map of oat. Five markers were placed on linkage groups corresponding to Mrg12 on the Avena sativa consensus map.

Citing Articles

Identification of reference genes for gene expression assessment in Avena sativa under biotic stress triggered by Blumeria graminis.

Sowa S, Toporowska J, Paczos-Grzeda E Sci Rep. 2024; 14(1):30006.

PMID: 39622934 PMC: 11612269. DOI: 10.1038/s41598-024-81348-4.

Resistant or Susceptible? How Central European Oat ( L.) Cultivars React to f. sp. Infection.

Cieplak M, Okon S Plants (Basel). 2023; 12(22).

PMID: 38005722 PMC: 10675455. DOI: 10.3390/plants12223825.

Genetic mapping of the powdery mildew resistance gene Pm7 on oat chromosome 5D.

Brodfuhrer S, Mohler V, Stadlmeier M, Okon S, Beuch S, Mascher M Theor Appl Genet. 2023; 136(3):53.

PMID: 36913008 PMC: 10011287. DOI: 10.1007/s00122-023-04288-z.

Chromosomal Location of -A Novel Powdery Mildew Resistance Gene from .

Ociepa T, Okon S Genes (Basel). 2022; 13(12).

PMID: 36553676 PMC: 9778159. DOI: 10.3390/genes13122409.

Virulence Structure of Blumeria graminis f. sp. avenae Populations in Poland across 2014-2015.

Cieplak M, Terlecka K, Ociepa T, Zimowska B, Okon S Plant Pathol J. 2021; 37(2):115-123.

PMID: 33866754 PMC: 8053843. DOI: 10.5423/PPJ.OA.10.2020.0193.

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