Effect of Lactoferrin on the Expression Profiles of Long Non-coding RNA During Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 Oct 2

PMID 31569432

Citations 8

Authors

Yan Xu

Jing-Jing An

Dina Tabys

Yin-Dan Xie

Tian-Yu Zhao

Hao-Wei Ren

Ning Liu

Affiliations

Soon will be listed here.

Abstract

Lactoferrin (LF) has demonstrated stimulation of osteogenic differentiation of mesenchymal stem cells (MSCs). Long non-coding RNAs (lncRNAs) participate in regulating the osteogenic differentiation processes. However, the impact of LF on lncRNA expression in MSC osteogenic differentiation is poorly understood. Our aim was to investigate the effects of LF on lncRNAs expression profiles, during osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs), by RNA sequencing. A total number of 1331 putative lncRNAs were identified in rBMSCs during osteogenic differentiation in the study. LF influenced the expression of 120 lncRNAs (differentially expressed lncRNAs [DELs], Fold change > 1.5 or < -1.5; < 0.05) in rBMSCs on day 14 of osteogenic differentiation, consisted of 60 upregulated and 60 down-regulated. Furthermore, the potential functions of DELs were of prediction by searching their target cis- and trans-regulated protein-coding genes. The bioinformatic analysis of DELs target gene revealed that LF led to the disfunction of transforming growth factor beta stimulus (TGF-β) and positive regulation of I-κappa B kinase/NF-κappa B signaling pathway, which may relate to osteogenic differentiation of rBMSCs. Our work is the first profiling of lncRNA in osteogenic differentiation of rBMSCs induced by LF, and provides valuable insights into the potential mechanisms for LF promoting osteogenic activity.

Citing Articles

lncRNA CASC11 regulates the progress of delayed fracture healing via sponging miR-150-3p.

Wu X, Shen T, Ji W, Huang M, Sima J, Li J J Orthop Surg Res. 2024; 19(1):757.

PMID: 39543626 PMC: 11562309. DOI: 10.1186/s13018-024-05226-5.

Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials-Induced Bone Fusion in a Rat Model of Lumbar Spinal Fusion.

Noh S, Sung K, Byeon H, Kim S, Kim K Int J Mol Sci. 2023; 24(21).

PMID: 37958766 PMC: 10649596. DOI: 10.3390/ijms242115782.

The role of lactoferrin in bone remodeling: evaluation of its potential in targeted delivery and treatment of metabolic bone diseases and orthopedic conditions.

Tian M, Han Y, Yang G, Li J, Shi C, Tian D Front Endocrinol (Lausanne). 2023; 14:1218148.

PMID: 37680888 PMC: 10482240. DOI: 10.3389/fendo.2023.1218148.

Lactoferrin Mediates Enhanced Osteogenesis of Adipose-Derived Stem Cells: Innovative Molecular and Cellular Therapy for Bone Repair.

Chang Y, Ping A, Chang C, Betz V, Cai L, Ren B Int J Mol Sci. 2023; 24(2).

PMID: 36675267 PMC: 9864243. DOI: 10.3390/ijms24021749.

Three-Dimensional Printing of Calcium Carbonate/Hydroxyapatite Scaffolds at Low Temperature for Bone Tissue Engineering.

Wang T, Zheng J, Hu T, Zhang H, Fu K, Yin R 3D Print Addit Manuf. 2023; 8(1):1-13.

PMID: 36655178 PMC: 9828593. DOI: 10.1089/3dp.2020.0140.

References

Viti F, Landini M, Mezzelani A, Petecchia L, Milanesi L, Scaglione S . Osteogenic Differentiation of MSC through Calcium Signaling Activation: Transcriptomics and Functional Analysis. PLoS One. 2016; 11(2):e0148173. PMC: 4734718. DOI: 10.1371/journal.pone.0148173. View

Xu Z, Chen H, Wang Z, Fan F, Shi P, Tu M . Isolation and Characterization of Peptides from Mytilus edulis with Osteogenic Activity in Mouse MC3T3-E1 Preosteoblast Cells. J Agric Food Chem. 2019; 67(5):1572-1584. DOI: 10.1021/acs.jafc.8b06530. View

Li Y, Zhang W, Ren F, Guo H . Activation of TGF-β Canonical and Noncanonical Signaling in Bovine Lactoferrin-Induced Osteogenic Activity of C3H10T1/2 Mesenchymal Stem Cells. Int J Mol Sci. 2019; 20(12). PMC: 6627184. DOI: 10.3390/ijms20122880. View

Wlodarski K . Lactoferrin--a promising bone-growth promoting milk-derived glycoprotein. Chir Narzadow Ruchu Ortop Pol. 2010; 74(5):257-9,322-3. View

Feng L, Xue D, Chen E, Zhang W, Gao X, Yu J . HMGB1 promotes the secretion of multiple cytokines and potentiates the osteogenic differentiation of mesenchymal stem cells through the Ras/MAPK signaling pathway. Exp Ther Med. 2017; 12(6):3941-3947. PMC: 5228376. DOI: 10.3892/etm.2016.3857. View

Peng X, Zhang Y, Wang Y, He Q, Yu Q . IGF-1 and BMP-7 synergistically stimulate articular cartilage repairing in the rabbit knees by improving chondrogenic differentiation of bone-marrow mesenchymal stem cells. J Cell Biochem. 2018; 120(4):5570-5582. DOI: 10.1002/jcb.27841. View

Zhu X, Yu J, Du J, Zhong G, Qiao L, Lin J . LncRNA HOXA-AS2 positively regulates osteogenesis of mesenchymal stem cells through inactivating NF-κB signalling. J Cell Mol Med. 2018; 23(2):1325-1332. PMC: 6349193. DOI: 10.1111/jcmm.14034. View

Takayama Y, Mizumachi K . Effect of lactoferrin-embedded collagen membrane on osteogenic differentiation of human osteoblast-like cells. J Biosci Bioeng. 2009; 107(2):191-5. DOI: 10.1016/j.jbiosc.2008.09.018. View

Jiang H, Hong T, Wang T, Wang X, Cao L, Xu X . Gene expression profiling of human bone marrow mesenchymal stem cells during osteogenic differentiation. J Cell Physiol. 2018; 234(5):7070-7077. DOI: 10.1002/jcp.27461. View

10.

Ma Q, Li L, Tang Y, Fu Q, Liu S, Hu S . Analyses of long non-coding RNAs and mRNA profiling through RNA sequencing of MDBK cells at different stages of bovine viral diarrhea virus infection. Res Vet Sci. 2017; 115:508-516. DOI: 10.1016/j.rvsc.2017.09.020. View

11.

Zhang W, Guo H, Jing H, Li Y, Wang X, Zhang H . Lactoferrin stimulates osteoblast differentiation through PKA and p38 pathways independent of lactoferrin's receptor LRP1. J Bone Miner Res. 2014; 29(5):1232-43. DOI: 10.1002/jbmr.2116. View

12.

Peng S, Cao L, He S, Zhong Y, Ma H, Zhang Y . An Overview of Long Noncoding RNAs Involved in Bone Regeneration from Mesenchymal Stem Cells. Stem Cells Int. 2018; 2018:8273648. PMC: 5829309. DOI: 10.1155/2018/8273648. View

13.

Love M, Huber W, Anders S . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014; 15(12):550. PMC: 4302049. DOI: 10.1186/s13059-014-0550-8. View

14.

Zhang W, Dong R, Diao S, Du J, Fan Z, Wang F . Differential long noncoding RNA/mRNA expression profiling and functional network analysis during osteogenic differentiation of human bone marrow mesenchymal stem cells. Stem Cell Res Ther. 2017; 8(1):30. PMC: 5297123. DOI: 10.1186/s13287-017-0485-6. View

15.

Deng L, Hong H, Zhang X, Chen D, Chen Z, Ling J . Down-regulated lncRNA MEG3 promotes osteogenic differentiation of human dental follicle stem cells by epigenetically regulating Wnt pathway. Biochem Biophys Res Commun. 2018; 503(3):2061-2067. DOI: 10.1016/j.bbrc.2018.07.160. View

16.

Trapnell C, Roberts A, Goff L, Pertea G, Kim D, Kelley D . Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nat Protoc. 2012; 7(3):562-78. PMC: 3334321. DOI: 10.1038/nprot.2012.016. View

17.

Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A . Effect of hydroxyapatite nanocrystals functionalized with lactoferrin in osteogenic differentiation of mesenchymal stem cells. J Biomed Mater Res A. 2014; 103(1):224-34. DOI: 10.1002/jbm.a.35170. View

18.

Molchadsky A, Shats I, Goldfinger N, Pevsner-Fischer M, Olson M, Rinon A . p53 plays a role in mesenchymal differentiation programs, in a cell fate dependent manner. PLoS One. 2008; 3(11):e3707. PMC: 2577894. DOI: 10.1371/journal.pone.0003707. View

19.

Li W, Zhu S, Hu J . Bone Regeneration Is Promoted by Orally Administered Bovine Lactoferrin in a Rabbit Tibial Distraction Osteogenesis Model. Clin Orthop Relat Res. 2015; 473(7):2383-93. PMC: 4457759. DOI: 10.1007/s11999-015-4270-5. View

20.

Cao B, Liu N, Wang W . High glucose prevents osteogenic differentiation of mesenchymal stem cells via lncRNA AK028326/CXCL13 pathway. Biomed Pharmacother. 2016; 84:544-551. DOI: 10.1016/j.biopha.2016.09.058. View