Mildew Resistance Locus O Genes and Are Negative Modulators of the Defense Response to

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 Sep 29

PMID 31561602

Citations 6

Authors

Guangchao Yu

Qiumin Chen

Xiangyu Wang

Xiangnan Meng

Yang Yu

Haiyan Fan

Na Cui

Affiliations

Soon will be listed here.

Abstract

Corynespora leaf spot caused by is one of the major diseases in cucumber ( L.). However, the resistance mechanisms and signals of cucumber to are unclear. Here, we report that the mildew resistance locus O (MLO) genes, and , are both negative modulators of the cucumber defense response to . Subcellular localization analysis showed that CsMLO1 and CsMLO2 are localized in the plasma membrane. Expression analysis indicated that the transcript levels of and are linked to the defense response to . Transient overexpression of either or in cucumber cotyledons reduced resistance to , whereas silencing of either or enhanced resistance to . The relationships of pathogenesis-related proteins, reactive oxygen species (ROS)-associated genes, and abscisic acid (ABA)-related genes to the overexpression and silencing of / in non-infested cucumber plants were investigated. The results indicated that mediated resistance against by regulating the expression of pathogenesis-related proteins and ROS-associated genes, as well as through ABA signaling pathway-associated genes. The -mediated resistance against primarily involves regulation of the expression of pathogenesis-related proteins. Our findings will guide strategies to enhance the resistance of cucumber to corynespora leaf spot.

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