A Novel Construct with Biomechanical Flexibility for Articular Cartilage Regeneration

Overview

Journal Stem Cell Res Ther

Publisher Biomed Central

Date 2019 Sep 25

PMID 31547887

Citations 13

Authors

Baixiang Cheng

Teng Tu

Xiao Shi

Yanzheng Liu

Ying Zhao

Yinhua Zhao

Yijie Li

Hui Chen

Yongjin Chen

Min Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Although tissue-engineered cartilage has been broadly studied, complete integration of regenerated cartilage with residual cartilage is still difficult for the inferior mechanical and biochemical feature of neocartilage. Chondrogenesis of mesenchymal stem cells can be induced by biophysical and biochemical factors.

Methods: In this study, autologous platelet-rich fibrin (PRF) membrane was used as a growth factor-rich scaffold that may facilitate differentiation of the transplanted bone marrow mesenchymal stem cells (BMSCs). At the same time, hydrostatic pressure was adopted for pre-adjustment of the seed cells before transplantation that may promote the mechanical flexibility of neocartilage.

Results: An in vitro study showed that the feasible hydrostatic pressure stimulation substantially promoted the chondrogenic potential of in vitro-cultured BMSC/PRF construct. In vivo results revealed that at every time point, the newborn tissues were the most favorable in the pressure-pretreated BMSC/PRF transplant group. Besides, the transplantation of feasible hydrostatic pressure-pretreated construct by BMSC sheet fragments and PRF granules could obviously improve the integration between the regenerated cartilage and host cartilage milieu, and thereby achieve boundaryless repair between the neocartilage and residual host cartilage tissue in rabbit temporomandibular joints. It could be concluded that feasible hydrostatic pressure may effectively promote the proliferation and chondrogenic differentiation of BMSCs in a BMSC/PRF construct.

Conclusion: This newly formed construct with biomechanical flexibility showed a superior capacity for cartilage regeneration by promoting the mechanical properties and integration of neocartilage.

Citing Articles

Laser Ablation Facilitates Implantation of Dynamic Self-Regenerating Cartilage for Articular Cartilage Regeneration.

Fan Y, Guastaldi F, Runyan G, Wang Y, Farinelli W, Randolph M J Funct Biomater. 2024; 15(6).

PMID: 38921522 PMC: 11204995. DOI: 10.3390/jfb15060148.

Mesenchymal Stem Cell-Based Therapies for Temporomandibular Joint Repair: A Systematic Review of Preclinical Studies.

Jiang Y, Shi J, Di W, Teo K, Toh W Cells. 2024; 13(11.

PMID: 38891122 PMC: 11171901. DOI: 10.3390/cells13110990.

Mechanochemical coupling of MGF mediates periodontal regeneration.

Zhao Y, Zhang S, Cheng B, Feng F, Zhu Y, Liu Y Bioeng Transl Med. 2024; 9(1):e10603.

PMID: 38193124 PMC: 10771565. DOI: 10.1002/btm2.10603.

Double-edged role of mechanical stimuli and underlying mechanisms in cartilage tissue engineering.

Jia Y, Le H, Wang X, Zhang J, Liu Y, Ding J Front Bioeng Biotechnol. 2023; 11:1271762.

PMID: 38053849 PMC: 10694366. DOI: 10.3389/fbioe.2023.1271762.

Bioadhesive and Injectable Hydrogels and Their Correlation with Mesenchymal Stem Cells Differentiation for Cartilage Repair: A Mini-Review.

Kovac J, Priscakova P, Gbelcova H, Heydari A, Ziaran S Polymers (Basel). 2023; 15(21).

PMID: 37959908 PMC: 10648146. DOI: 10.3390/polym15214228.

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