Prevalence and Genotyping of Infected to DsRNA Virus by PCR-Restriction Fragment Length Polymorphism (RFLP)

Overview

Journal Iran J Parasitol

Publisher Tehran University of Medical Sciences

Specialty Parasitology

Date 2019 Sep 24

PMID 31543913

Citations 3

Authors

Fariba Orujzadeh

Fatemeh Tabatabaie

Khadijeh Khanaliha

Lame Akhlaghi

Farah Bokharaei-Salim

Soudabeh Fallah

Abdoulreza Esteghamati

Hossein Masoumi-Asl

Affiliations

Soon will be listed here.

Abstract

Background: is a prevalent sexually transmitted infection cause trichomoniasis. In this study prevalence and genotype of Iranian isolates of infected (dsRNA) viruses were evaluated by PCR-RFLP and obtained patterns were then confirmed by sequence analysis and genotype of these Iranian isolates confirmed again.

Methods: Ten strains of were collected from 1700 vaginal samples of women referred to hospitals associated with Iran University of Medical Sciences in Tehran, Iran during Feb 2016 to Jul 2017, evaluated in points of infection to Virus (TVV-1) were used in a PCR-RFLP. All of ten isolates of T. vaginalis were examined by designed nested PCR for actin gene and then digestion patterns of three endonuclease enzymes of HindII, MseI and RsaI were evaluated and genotype of these isolates was defined.

Results: By combination of fragments pattern of three enzymes of HindII, RsaI and MseI, three genotypes were found; six genotypes E, two genotypes G and two genotypes I. The most dominant genotypes were genotype E. Among four TVV infected isolates two genotype E, one genotype G and one genotype I were found, however among six uninfected isolates to TVV-1, all of three genotypes were also found.

Conclusion: Three genotypes E, G and I in infected with dsRNA isolates were found, however, these three genotypes in without virus were also observed. Further study is needed to evaluate genotypes of , which infected virus in more great T. vaginalis population.

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