High Genetic Diversity and Species Complexity of Associated With Grapevine Dieback in China

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Sep 24

PMID 31543868

Citations 40

Authors

Ishara S Manawasinghe

Asha J Dissanayake

Xinghong Li

Mei Liu

Dhanushka N Wanasinghe

Jianping Xu

Wensheng Zhao

Wei Zhang

Yueyan Zhou

Kevin D Hyde

Siraprapa Brooks

Jiye Yan

Affiliations

Soon will be listed here.

Abstract

Grapevine trunk diseases have become one of the main threats to grape production worldwide, with species as an emerging group of pathogens in China. At present, relatively little is known about the taxonomy and genetic diversity of Chinese populations, including their relationships to other populations worldwide. Here, we conducted an extensive field survey in six provinces in China to identify and characterize species in grape vineyards. Ninety-four isolates were identified and analyzed using multi-locus phylogeny. The isolates belonged to eight species, including three novel taxa, , and three new host records, , and . The most commonly isolated species was . In addition, high genetic diversity was observed for in Chinese vineyards. Haplotype network analysis of isolates from China and Europe showed a close relationship between samples from the two geographical locations and evidence for recombination. In comparative pathogenicity testing, was the most aggressive taxon, whereas was the least aggressive. This study provides new insights into the species associated with grapevines in China, and our results can be used to develop effective disease management strategies.

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References

Bandelt H, Forster P, Rohl A . Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol. 1999; 16(1):37-48. DOI: 10.1093/oxfordjournals.molbev.a026036. View

Zhang W, Manawasinghe I, Zhao W, Xu J, Brooks S, Zhao X . Multiple gene genealogy reveals high genetic diversity and evidence for multiple origins of Chinese Plasmopara viticola population. Sci Rep. 2017; 7(1):17304. PMC: 5725484. DOI: 10.1038/s41598-017-17569-7. View

Santos J, Vrandecic K, Cosic J, Duvnjak T, Phillips A . Resolving the Diaporthe species occurring on soybean in Croatia. Persoonia. 2012; 27:9-19. PMC: 3251324. DOI: 10.3767/003158511X603719. View

Posada , Crandall . Intraspecific gene genealogies: trees grafting into networks. Trends Ecol Evol. 2001; 16(1):37-45. DOI: 10.1016/s0169-5347(00)02026-7. View

Wu H, Yang H, You X, Li Y . Isolation and characterization of saponin-producing fungal endophytes from Aralia elata in Northeast China. Int J Mol Sci. 2012; 13(12):16255-66. PMC: 3546688. DOI: 10.3390/ijms131216255. View

Guarnaccia V, Groenewald J, Woodhall J, Armengol J, Cinelli T, Eichmeier A . diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe. Persoonia. 2018; 40:135-153. PMC: 6146647. DOI: 10.3767/persoonia.2018.40.06. View

Bai Q, Zhai L, Chen X, Hong N, Xu W, Wang G . Biological and Molecular Characterization of Five Phomopsis Species Associated with Pear Shoot Canker in China. Plant Dis. 2019; 99(12):1704-1712. DOI: 10.1094/PDIS-03-15-0259-RE. View

Gramaje D, Armengol J . Fungal Trunk Pathogens in the Grapevine Propagation Process: Potential Inoculum Sources, Detection, Identification, and Management Strategies. Plant Dis. 2019; 95(9):1040-1055. DOI: 10.1094/PDIS-01-11-0025. View

Stamatakis A, Hoover P, Rougemont J . A rapid bootstrap algorithm for the RAxML Web servers. Syst Biol. 2008; 57(5):758-71. DOI: 10.1080/10635150802429642. View

10.

Udayanga D, Castlebury L, Rossman A, Chukeatirote E, Hyde K . The Diaporthe sojae species complex: Phylogenetic re-assessment of pathogens associated with soybean, cucurbits and other field crops. Fungal Biol. 2015; 119(5):383-407. DOI: 10.1016/j.funbio.2014.10.009. View

11.

Gao Y, Liu F, Duan W, Crous P, Cai L . is paraphyletic. IMA Fungus. 2017; 8(1):153-187. PMC: 5493532. DOI: 10.5598/imafungus.2017.08.01.11. View

12.

Lindahl J, Grace D . The consequences of human actions on risks for infectious diseases: a review. Infect Ecol Epidemiol. 2015; 5:30048. PMC: 4663196. DOI: 10.3402/iee.v5.30048. View

13.

Librado P, Rozas J . DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009; 25(11):1451-2. DOI: 10.1093/bioinformatics/btp187. View

14.

Senanayake I, Crous P, Groenewald J, Maharachchikumbura S, Jeewon R, Phillips A . Families of based on morphological and phylogenetic evidence. Stud Mycol. 2017; 86:217-296. PMC: 5603113. DOI: 10.1016/j.simyco.2017.07.003. View

15.

Lawrence D, Travadon R, Baumgartner K . Diversity of Diaporthe species associated with wood cankers of fruit and nut crops in northern California. Mycologia. 2015; 107(5):926-40. DOI: 10.3852/14-353. View

16.

Yan J, Zhao W, Chen Z, Xing Q, Zhang W, Chethana K . Comparative genome and transcriptome analyses reveal adaptations to opportunistic infections in woody plant degrading pathogens of Botryosphaeriaceae. DNA Res. 2017; 25(1):87-102. PMC: 5824938. DOI: 10.1093/dnares/dsx040. View

17.

Mondal S, Vicent A, Reis R, Timmer L . Saprophytic Colonization of Citrus Twigs by Diaporthe citri and Factors Affecting Pycnidial Production and Conidial Survival. Plant Dis. 2019; 91(4):387-392. DOI: 10.1094/PDIS-91-4-0387. View

18.

Mathew F, Alananbeh K, Jordahl J, Meyer S, Castlebury L, Gulya T . Phomopsis Stem Canker: A Reemerging Threat to Sunflower (Helianthus annuus) in the United States. Phytopathology. 2015; 105(7):990-7. DOI: 10.1094/PHYTO-11-14-0336-FI. View

19.

Huang F, Udayanga D, Wang X, Hou X, Mei X, Fu Y . Endophytic Diaporthe associated with Citrus: A phylogenetic reassessment with seven new species from China. Fungal Biol. 2015; 119(5):331-47. DOI: 10.1016/j.funbio.2015.02.006. View

20.

Kelly J . A test of neutrality based on interlocus associations. Genetics. 1997; 146(3):1197-206. PMC: 1208047. DOI: 10.1093/genetics/146.3.1197. View