Arabidopsis JANUS Regulates Embryonic Pattern Formation Through Pol II-Mediated Transcription of WOX2 and PIN7

Overview

Journal iScience

Publisher Cell Press

Date 2019 Sep 23

PMID 31542701

Citations 15

Authors

Feng Xiong

Hai-Hong Liu

Cun-Ying Duan

Bi-Ke Zhang

Guo Wei

Yan Zhang

Sha Li

Affiliations

Soon will be listed here.

Abstract

Embryonic pattern formation relies on positional coordination of cell division and specification. Early axis formation during Arabidopsis embryogenesis requires WUSCHEL RELATED HOMEOBOX (WOX)-mediated transcription activation and PIN-FORMED7 (PIN7)-mediated auxin asymmetry. How these events are regulated is obscure. We report that Arabidopsis JANUS, a putative subunit of spliceosome, is essential for embryonic pattern formation. Significantly reduced transcription but not mRNA processing of WOX2 and PIN7 in janus suggested its role in transcriptional regulation. JANUS interacts with RNA polymerase II (Pol II) through a region outside of its spliceosome-association domain. We further show that Pol II mediates the transcription of WOX2 and PIN7 in a JANUS-dependent way and is essential for embryonic pattern formation. These findings reveal that JANUS recruits Pol II for the activation of two parallel pathways to ensure proper pattern formation during embryogenesis.

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References

Yan Q, Xia X, Sun Z, Fang Y . Depletion of Arabidopsis SC35 and SC35-like serine/arginine-rich proteins affects the transcription and splicing of a subset of genes. PLoS Genet. 2017; 13(3):e1006663. PMC: 5362245. DOI: 10.1371/journal.pgen.1006663. View

Xiao R, Sun Y, Ding J, Lin S, Rose D, Rosenfeld M . Splicing regulator SC35 is essential for genomic stability and cell proliferation during mammalian organogenesis. Mol Cell Biol. 2007; 27(15):5393-402. PMC: 1952092. DOI: 10.1128/MCB.00288-07. View

Kershnar E, Wu S, Chiang C . Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes. J Biol Chem. 1998; 273(51):34444-53. DOI: 10.1074/jbc.273.51.34444. View

Kanno T, Huettel B, Mette M, Aufsatz W, Jaligot E, Daxinger L . Atypical RNA polymerase subunits required for RNA-directed DNA methylation. Nat Genet. 2005; 37(7):761-5. DOI: 10.1038/ng1580. View

Werner F, Grohmann D . Evolution of multisubunit RNA polymerases in the three domains of life. Nat Rev Microbiol. 2011; 9(2):85-98. DOI: 10.1038/nrmicro2507. View

Meinke D, Muralla R, Sweeney C, Dickerman A . Identifying essential genes in Arabidopsis thaliana. Trends Plant Sci. 2008; 13(9):483-91. DOI: 10.1016/j.tplants.2008.06.003. View

Onodera Y, Nakagawa K, Haag J, Pikaard D, Mikami T, Ream T . Sex-biased lethality or transmission of defective transcription machinery in Arabidopsis. Genetics. 2008; 180(1):207-18. PMC: 2535675. DOI: 10.1534/genetics.108.090621. View

Thomas M, Chiang C . The general transcription machinery and general cofactors. Crit Rev Biochem Mol Biol. 2006; 41(3):105-78. DOI: 10.1080/10409230600648736. View

Ream T, Haag J, Pontvianne F, Nicora C, Norbeck A, Pasa-Tolic L . Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit. Nucleic Acids Res. 2015; 43(8):4163-78. PMC: 4417161. DOI: 10.1093/nar/gkv247. View

10.

Onodera Y, Haag J, Ream T, Costa Nunes P, Pontes O, Pikaard C . Plant nuclear RNA polymerase IV mediates siRNA and DNA methylation-dependent heterochromatin formation. Cell. 2005; 120(5):613-22. DOI: 10.1016/j.cell.2005.02.007. View

11.

Braunschweig U, Gueroussov S, Plocik A, Graveley B, Blencowe B . Dynamic integration of splicing within gene regulatory pathways. Cell. 2013; 152(6):1252-69. PMC: 3642998. DOI: 10.1016/j.cell.2013.02.034. View

12.

Robert H, Park C, Gutierrez C, Wojcikowska B, Pencik A, Novak O . Maternal auxin supply contributes to early embryo patterning in Arabidopsis. Nat Plants. 2018; 4(8):548-553. PMC: 6076996. DOI: 10.1038/s41477-018-0204-z. View

13.

Dolan W, Chapple C . Conservation and Divergence of Mediator Structure and Function: Insights from Plants. Plant Cell Physiol. 2017; 58(1):4-21. DOI: 10.1093/pcp/pcw176. View

14.

Zhang Z, Tucker E, Hermann M, Laux T . A Molecular Framework for the Embryonic Initiation of Shoot Meristem Stem Cells. Dev Cell. 2017; 40(3):264-277.e4. DOI: 10.1016/j.devcel.2017.01.002. View

15.

van Roon A, Oubridge C, Obayashi E, Sposito B, Newman A, Seraphin B . Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p-binding domain. RNA. 2017; 23(6):968-981. PMC: 5435868. DOI: 10.1261/rna.059378.116. View

16.

Wu H, Sun S, Tu K, Gao Y, Xie B, Krainer A . A splicing-independent function of SF2/ASF in microRNA processing. Mol Cell. 2010; 38(1):67-77. PMC: 3395997. DOI: 10.1016/j.molcel.2010.02.021. View

17.

Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park J, Kim S . STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis. Nucleic Acids Res. 2012; 41(3):1984-97. PMC: 3561960. DOI: 10.1093/nar/gks1309. View

18.

Bentley D . The mRNA assembly line: transcription and processing machines in the same factory. Curr Opin Cell Biol. 2002; 14(3):336-42. DOI: 10.1016/s0955-0674(02)00333-2. View

19.

Lau S, Slane D, Herud O, Kong J, Jurgens G . Early embryogenesis in flowering plants: setting up the basic body pattern. Annu Rev Plant Biol. 2012; 63:483-506. DOI: 10.1146/annurev-arplant-042811-105507. View

20.

Cramer P, Armache K, Baumli S, Benkert S, Brueckner F, Buchen C . Structure of eukaryotic RNA polymerases. Annu Rev Biophys. 2008; 37:337-52. DOI: 10.1146/annurev.biophys.37.032807.130008. View