Diallyl Trisulfide Protects Rat Brain Tissue Against the Damage Induced by Ischemia-Reperfusion Through the Nrf2 Pathway

Overview

Journal Antioxidants (Basel)

Date 2019 Sep 22

PMID 31540440

Citations 12

Authors

Carlos A Silva-Islas

Maria E Chanez-Cardenas

Diana Barrera-Oviedo

Alma Ortiz-Plata

Jose Pedraza-Chaverri

Perla D Maldonado

Affiliations

Soon will be listed here.

Abstract

Stroke is a public health problem due to its high mortality and disability rates; despite these, the pharmacological treatments are limited. Oxidative stress plays an important role in cerebral damage in stroke and the activation of the nuclear factor erythroid 2-related factor 2 (Nrf2) confers protection against oxidative stress. Different compounds, such as diallyl trisulfide (DATS), have the ability to activate Nrf2. DATS protects against the damage induced in oxygen-glucose deprivation in neuronal cells; however, in in vivo models of cerebral ischemia, DATS has not been evaluated. Male Wistar rats were subjected to 1 h of ischemia and seven days of reperfusion and the protective effect of DATS was evaluated. DATS administration (IR + DATS) decreased the infarct area and brain damage in the striatum and cortex; improved neurological function; decreased malondialdehyde and metalloproteinase-9 levels; increased Nrf2 activation in the cortex and the expression of superoxide dismutase 1 (SOD1) in the nucleus, SOD2 and glutathione S-transferase (GST) in the striatum and cortex; and increased the activity of catalase (CAT) in the striatum and glutathione peroxidase (GPx) in the cortex. Our results demonstrate the protective effect of DATS in an in vivo model of cerebral ischemia that involves Nrf2 activation.

Citing Articles

Neuroprotective Effects of [Formula: see text]-Terpinene in Rats with Acute Cerebral Ischemia: Modulation of Inflammation, Apoptosis, and Oxidation.

Khoshnazar S, Kazemi M, Amirheidari B Neurochem Res. 2024; 49(7):1863-1878.

PMID: 38753259 DOI: 10.1007/s11064-024-04143-7.

Insights on Natural Products Against Amyotrophic Lateral Sclerosis (ALS).

Monteiro K, Alcantara M, Aquino T, Silva-Junior E Curr Neuropharmacol. 2024; 22(7):1169-1188.

PMID: 38708921 PMC: 10964095. DOI: 10.2174/1570159X22666231016153606.

Antioxidant and Anti-Inflammatory Effects of Garlic in Ischemic Stroke: Proposal of a New Mechanism of Protection through Regulation of Neuroplasticity.

Bautista-Perez S, Silva-Islas C, Sandoval-Marquez O, Toledo-Toledo J, Bello-Martinez J, Barrera-Oviedo D Antioxidants (Basel). 2023; 12(12).

PMID: 38136245 PMC: 10740829. DOI: 10.3390/antiox12122126.

The anti-inflammatory effect of dimethyl trisulfide in experimental acute pancreatitis.

Orjan E, Kormanyos E, Mihalekne Fur G, Dombi A, Balint E, Balla Z Sci Rep. 2023; 13(1):16813.

PMID: 37798377 PMC: 10556037. DOI: 10.1038/s41598-023-43692-9.

Reactive Sulfur Species Omics Analysis in the Brain Tissue of the 5xFAD Mouse Model of Alzheimer's Disease.

Kinno A, Kasamatsu S, Akaike T, Ihara H Antioxidants (Basel). 2023; 12(5).

PMID: 37237971 PMC: 10215359. DOI: 10.3390/antiox12051105.

References

Miltonprabu S, Sumedha N, Senthilraja P . Diallyl trisulfide, a garlic polysulfide protects against As-induced renal oxidative nephrotoxicity, apoptosis and inflammation in rats by activating the Nrf2/ARE signaling pathway. Int Immunopharmacol. 2017; 50:107-120. DOI: 10.1016/j.intimp.2017.06.011. View

Lakhan S, Kirchgessner A, Hofer M . Inflammatory mechanisms in ischemic stroke: therapeutic approaches. J Transl Med. 2009; 7:97. PMC: 2780998. DOI: 10.1186/1479-5876-7-97. View

Sun M, Bu H, Li B, Yu J, Guo Y, Li C . Neuroprotective potential of phase II enzyme inducer diallyl trisulfide. Neurol Res. 2008; 31(1):23-7. DOI: 10.1179/174313208X332959. View

Fisher C, Augustine L, Maher J, Nelson D, Slitt A, Klaassen C . Induction of drug-metabolizing enzymes by garlic and allyl sulfide compounds via activation of constitutive androstane receptor and nuclear factor E2-related factor 2. Drug Metab Dispos. 2007; 35(6):995-1000. DOI: 10.1124/dmd.106.014340. View

Ali M, Al-Qattan K, Khanafer R, Mustafa T . Effect of allicin from garlic powder on serum lipids and blood pressure in rats fed with a high cholesterol diet. Prostaglandins Leukot Essent Fatty Acids. 2000; 62(4):253-9. DOI: 10.1054/plef.2000.0152. View

Xu X, Li G, Wang B, Qin Y, Bai S, Rong J . Diallyl trisufide protects against oxygen glucose deprivation -induced apoptosis by scavenging free radicals via the PI3K/Akt -mediated Nrf2/HO-1 signaling pathway in B35 neural cells. Brain Res. 2015; 1614:38-50. DOI: 10.1016/j.brainres.2015.04.014. View

Guo Y, Zhang K, Wang Q, Li Z, Yin Y, Xu Q . Neuroprotective effects of diallyl trisulfide in SOD1-G93A transgenic mouse model of amyotrophic lateral sclerosis. Brain Res. 2010; 1374:110-5. DOI: 10.1016/j.brainres.2010.12.014. View

Tebay L, Robertson H, Durant S, Vitale S, Penning T, Dinkova-Kostova A . Mechanisms of activation of the transcription factor Nrf2 by redox stressors, nutrient cues, and energy status and the pathways through which it attenuates degenerative disease. Free Radic Biol Med. 2015; 88(Pt B):108-146. PMC: 4659505. DOI: 10.1016/j.freeradbiomed.2015.06.021. View

Papp D, Lenti K, Modos D, Fazekas D, Dul Z, Turei D . The NRF2-related interactome and regulome contain multifunctional proteins and fine-tuned autoregulatory loops. FEBS Lett. 2012; 586(13):1795-802. PMC: 7511993. DOI: 10.1016/j.febslet.2012.05.016. View

10.

Skiljic D, Petersen A, Karlsson J, Behndig A, Nilsson S, Zetterberg M . Effects of 17β-Estradiol on Activity, Gene and Protein Expression of Superoxide Dismutases in Primary Cultured Human Lens Epithelial Cells. Curr Eye Res. 2018; 43(5):639-646. DOI: 10.1080/02713683.2018.1437923. View

11.

Gupta R, Singh M, Sharma A . Neuroprotective effect of antioxidants on ischaemia and reperfusion-induced cerebral injury. Pharmacol Res. 2003; 48(2):209-15. DOI: 10.1016/s1043-6618(03)00102-6. View

12.

Zhang D, Lo S, Cross J, Templeton D, Hannink M . Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex. Mol Cell Biol. 2004; 24(24):10941-53. PMC: 533977. DOI: 10.1128/MCB.24.24.10941-10953.2004. View

13.

Siket M . Treatment of Acute Ischemic Stroke. Emerg Med Clin North Am. 2016; 34(4):861-882. DOI: 10.1016/j.emc.2016.06.009. View

14.

Shin S, Song J, Hwang B, Lyea Park S, Kim W, Park S . Angiopoietin-like protein 4 potentiates DATS-induced inhibition of proliferation, migration, and invasion of bladder cancer EJ cells; involvement of G/M-phase cell cycle arrest, signaling pathways, and transcription factors-mediated MMP-9 expression. Food Nutr Res. 2017; 61(1):1338918. PMC: 5492081. DOI: 10.1080/16546628.2017.1338918. View

15.

Fukushima S, Takada N, Hori T, Wanibuchi H . Cancer prevention by organosulfur compounds from garlic and onion. J Cell Biochem Suppl. 1997; 27:100-5. View

16.

Guo Z, Kozlov S, Lavin M, Person M, Paull T . ATM activation by oxidative stress. Science. 2010; 330(6003):517-21. DOI: 10.1126/science.1192912. View

17.

Zeng T, Zhang C, Zhu Z, Yu L, Zhao X, Xie K . Diallyl trisulfide (DATS) effectively attenuated oxidative stress-mediated liver injury and hepatic mitochondrial dysfunction in acute ethanol-exposed mice. Toxicology. 2008; 252(1-3):86-91. DOI: 10.1016/j.tox.2008.07.062. View

18.

Longa E, Weinstein P, Carlson S, Cummins R . Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 1989; 20(1):84-91. DOI: 10.1161/01.str.20.1.84. View

19.

Motohashi H, Katsuoka F, Engel J, Yamamoto M . Small Maf proteins serve as transcriptional cofactors for keratinocyte differentiation in the Keap1-Nrf2 regulatory pathway. Proc Natl Acad Sci U S A. 2004; 101(17):6379-84. PMC: 404053. DOI: 10.1073/pnas.0305902101. View

20.

Wu C, Sheen L, Chen H, Kuo W, Tsai S, Lii C . Differential effects of garlic oil and its three major organosulfur components on the hepatic detoxification system in rats. J Agric Food Chem. 2002; 50(2):378-83. DOI: 10.1021/jf010937z. View