Impact of the Lipopolysaccharide Chemotype of Serovar Typhimurium on Virulence in Gnotobiotic Piglets

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2019 Sep 22

PMID 31540295

Citations 6

Authors

Alla Splichalova

Zdislava Splichalova

Daniela Karasova

Ivan Rychlik

Paolo Trevisi

Marek Sinkora

Igor Splichal

Affiliations

Soon will be listed here.

Abstract

Typhimurium is an enteric pathogen that causes acute and chronic infections in humans and animals. One-week-old germ-free piglets were orally colonized/infected with the Typhimurium LT2 strain or its isogenic rough Δ, Δ or Δ mutants with exactly defined lipopolysaccharide (LPS) defects. After 24 h, the piglets were euthanized and the colonization of the small intestine, translocations into the mesenteric lymph nodes, liver, spleen, lungs, and bacteremia, along with changes in the ileum histology, and transcription levels of the tight junction proteins claudin-1, claudin-2, and occludin were all assessed. Additionally, transcription levels of IL-8, TNF-α, and IL-10 in the terminal ileum, and their local and systemic protein levels were evaluated. Wild-type Typhimurium showed the highest translocation, histopathological changes, upregulation of claudins and downregulation of occludin, transcription of the cytokines, intestinal IL-8 and TNF-α levels, and systemic TNF-α and IL-10 levels. Depending on the extent of the incompleteness of the LPS, the levels of the respective elements decreased, or no changes were observed at all in the piglets colonized/infected with Δ mutants. Intestinal IL-10 and systemic IL-8 levels were not detected in any piglet groups. This study provided foundational data on the gnotobiotic piglet response to colonization/infection with the exactly defined rough LT2 isogenic mutants.

Citing Articles

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Splichalova A, Kindlova Z, Killer J, Neuzil Bunesova V, Vlkova E, Valaskova B Pathogens. 2023; 12(11).

PMID: 38003758 PMC: 10675043. DOI: 10.3390/pathogens12111293.

SaaS sRNA promotes intestinal invasion via modulating MAPK inflammatory pathway.

Cai L, Xie Y, Shao L, Hu H, Xu X, Wang H Gut Microbes. 2023; 15(1):2211184.

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Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by subsp. BB-12 and Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants.

Splichal I, Donovan S, Kindlova Z, Stranak Z, Neuzil Bunesova V, Sinkora M Int J Mol Sci. 2023; 24(3).

PMID: 36768650 PMC: 9916534. DOI: 10.3390/ijms24032329.

Monoassociation of Preterm Germ-Free Piglets with Subsp. BB-12 and Its Impact on Infection with Typhimurium.

Splichalova A, Donovan S, Tlaskalova-Hogenova H, Stranak Z, Splichalova Z, Splichal I Biomedicines. 2021; 9(2).

PMID: 33670419 PMC: 7917597. DOI: 10.3390/biomedicines9020183.

Colonization of Germ-Free Piglets with Mucinolytic and Non-Mucinolytic Strains Isolated from the Intestine of Wild Boar and Their Interference with Typhimurium.

Splichalova A, Pechar R, Killer J, Splichalova Z, Neuzil Bunesova V, Vlkova E Microorganisms. 2020; 8(12).

PMID: 33333934 PMC: 7765441. DOI: 10.3390/microorganisms8122002.

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