Regulation of Hydrogen Sulfide Metabolism by Nitric Oxide Inhibitors and the Quality of Peaches During Cold Storage

Overview

Journal Antioxidants (Basel)

Date 2019 Sep 19

PMID 31527494

Citations 10

Authors

Biao Geng

Dandan Huang

Shuhua Zhu

Affiliations

Soon will be listed here.

Abstract

Both nitric oxide (NO) and hydrogen sulfide (HS) have been shown to have positive effects on the maintenance of fruit quality during storage; however, the mechanisms by which NO regulates the endogenous HS metabolism remain unknown. In this experiment, peaches were immersed in solutions of NO, potassium 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO, as an NO scavenger), N-nitro-l-arginine methyl ester (l-NAME, as an inhibitor of nitric oxide synthase (NOS)-like activity), and sodium tungstate (as an inhibitor of nitrate reductase), and the resulting changes in the HS metabolism of peaches were studied. The results showed that exogenous NO reduced the contents of endogenous HS, Cys, and sulfite; decreased the activities of l-/d-cysteine desulfhydrase (l-/d-CD), -acetylserine (thiol)lyase (OAS-TL), and sulfite reductase (SiR); and increased the activity of β-cyanoalanine synthase (β-CAS). Both c-PTIO and sodium tungstate had similar roles in increasing the HS content by sustaining the activities of l-/d-CDs, OAS-TL, and SiR. l-NAME increased the HS content, mainly by maintaining the d-CD activity. The results suggest that NO, c-PTIO, l-NAME, and sodium tungstate differently regulate the HS metabolism of peaches during storage.

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