Active Surveillance and Genetic Evolution of Avian Influenza Viruses in Egypt, 2016-2018

Overview

Journal Emerg Microbes Infect

Specialty Infectious Diseases

Date 2019 Sep 19

PMID 31526249

Citations 17

Authors

Ahmed Kandeil

Joseph T Hicks

Sean G Young

Ahmed N El Taweel

Ahmed S Kayed

Yassmin Moatasim

Omnia Kutkat

Ola Bagato

Pamela P McKenzie

Zhipeng Cai

Rebecca Badra

Mohamed Kutkat

Justin Bahl

Richard J Webby

Ghazi Kayali

Mohamed A Ali

Affiliations

Soon will be listed here.

Abstract

Egypt is a hotspot for avian influenza virus (AIV) due to the endemicity of H5N1 and H9N2 viruses. AIVs were isolated from 329 samples collected in 2016-2018; 48% were H9N2, 37.1% were H5N8, 7.6% were H5N1, and 7.3% were co-infections with 2 of the 3 subtypes. The 32 hemagglutinin (HA) sequences of the H5N1 viruses formed a well-defined lineage within clade 2.2.1.2. The 10 HA sequences of the H5N8 viruses belonged to a subclade within 2.3.4.4. The 11 HA of H9N2 isolates showed high sequence homology with other Egyptian G1-like H9N2 viruses. The prevalence of H5N8 viruses in ducks (2.4%) was higher than in chickens (0.94%). Genetic reassortment was detected in H9N2 viruses. Antigenic analysis showed that H9N2 viruses are homogenous, antigenic drift was detected among H5N1 viruses. AI H5N8 showed higher replication rate followed by H9N2 and H5N1, respectively. H5N8 was more common in Southern Egypt, H9N2 in the Nile Delta, and H5N1 in both areas. Ducks and chickens played a significant role in transmission of H5N1 viruses. The endemicity and co-circulation of H5N1, H5N8, and H9N2 AIV coupled with the lack of a clear control strategy continues to provide avenues for further virus evolution in Egypt.

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