Using Fluorescence Microscopy to Shed Light on the Mechanisms of Antimicrobial Peptides

Overview

Journal Future Med Chem

Specialties Chemistry
Pharmacy

Date 2019 Sep 14

PMID 31517514

Citations 5

Authors

Anne K Buck

Donald E Elmore

Louise Eo Darling

Affiliations

Soon will be listed here.

Abstract

Antimicrobial peptides (AMPs) are promising in the fight against increasing bacterial resistance, but the development of AMPs with enhanced activity requires a thorough understanding of their mechanisms of action. Fluorescence microscopy is one of the most flexible and effective tools to characterize AMPs, particularly in its ability to measure the membrane interactions and cellular localization of peptides. Recent advances have increased the scope of research questions that can be addressed via microscopy through improving spatial and temporal resolution. Unique combinations of fluorescent labels and dyes can simultaneously consider different aspects of peptide-membrane interaction mechanisms. This review emphasizes the central role that fluorescence microscopy will continue to play in the interrogation of AMP structure-function relationships and the engineering of more potent peptides.

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