Detection and Occurrence of Indicator Organisms and Pathogens

Overview

Journal Water Environ Res

Date 2019 Sep 11

PMID 31505073

Citations 13

Authors

Amir M Motlagh

Zhengjian Yang

Affiliations

Soon will be listed here.

Abstract

This review paper focuses on detection and quantification techniques of indicator organisms that can be used for water quality assessment. The environmental pathogens that are critical to understand and better evaluate water quality are also discussed in this paper. Several recent studies using culture-independent methods such as microbial source tracking, pulsed field gel electrophoresis, mitochondrial DNA, and next generation sequencing to assess various environmental samples and water bodies have been reviewed. PRACTITIONER POINTS: Various waterborne pathogens and cases of outbreak occurances due to presence of pathogens are studied in this review paper. Recent studies for detecting major indicator organisms to evaluate the presence of pathogens in water bodies are reviewed. Culture-independent techniques as robust tools to detect and quantify waterborne pathogens are discussed in this review paper.

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References

McLellan S, Eren A . Discovering new indicators of fecal pollution. Trends Microbiol. 2014; 22(12):697-706. PMC: 4256112. DOI: 10.1016/j.tim.2014.08.002. View

He X, Liu P, Zheng G, Chen H, Shi W, Cui Y . Evaluation of five microbial and four mitochondrial DNA markers for tracking human and pig fecal pollution in freshwater. Sci Rep. 2016; 6:35311. PMC: 5062121. DOI: 10.1038/srep35311. View

Moussa S, Massengale R . Identification of the sources of Escherichia coli in a watershed using carbon-utilization patterns and composite data sets. J Water Health. 2008; 6(2):197-207. DOI: 10.2166/wh.2008.021. View

Villemur R, Imbeau M, Vuong M, Masson L, Payment P . An environmental survey of surface waters using mitochondrial DNA from human, bovine and porcine origin as fecal source tracking markers. Water Res. 2014; 69:143-153. DOI: 10.1016/j.watres.2014.10.063. View

Lee J, Lee H, Cho Y, Hur H, Ko G . F+ RNA coliphage-based microbial source tracking in water resources of South Korea. Sci Total Environ. 2011; 412-413:127-31. DOI: 10.1016/j.scitotenv.2011.09.061. View

Tan B, Ng C, Nshimyimana J, Loh L, Gin K, Thompson J . Next-generation sequencing (NGS) for assessment of microbial water quality: current progress, challenges, and future opportunities. Front Microbiol. 2015; 6:1027. PMC: 4585245. DOI: 10.3389/fmicb.2015.01027. View

Kirs M, Caffaro-Filho R, Wong M, Harwood V, Moravcik P, Fujioka R . Human-Associated Bacteroides spp. and Human Polyomaviruses as Microbial Source Tracking Markers in Hawaii. Appl Environ Microbiol. 2016; 82(22):6757-6767. PMC: 5086569. DOI: 10.1128/AEM.01959-16. View

Park J, Kim J, Kim S, Shin E, Oh K, Kim Y . A waterborne outbreak of multiple diarrhoeagenic Escherichia coli infections associated with drinking water at a school camp. Int J Infect Dis. 2017; 66:45-50. DOI: 10.1016/j.ijid.2017.09.021. View

Costa A, Gusmara C, Gardoni D, Zaninelli M, Tambone F, Sala V . The effect of anaerobic digestion and storage on indicator microorganisms in swine and dairy manure. Environ Sci Pollut Res Int. 2017; 24(31):24135-24146. DOI: 10.1007/s11356-017-0011-5. View

10.

Yamahara K, Sassoubre L, Goodwin K, Boehm A . Occurrence and persistence of bacterial pathogens and indicator organisms in beach sand along the California coast. Appl Environ Microbiol. 2012; 78(6):1733-45. PMC: 3298156. DOI: 10.1128/AEM.06185-11. View

11.

Cui Q, Fang T, Huang Y, Dong P, Wang H . Evaluation of bacterial pathogen diversity, abundance and health risks in urban recreational water by amplicon next-generation sequencing and quantitative PCR. J Environ Sci (China). 2017; 57:137-149. DOI: 10.1016/j.jes.2016.11.008. View

12.

Waso M, Khan S, Khan W . Development and small-scale validation of a novel pigeon-associated mitochondrial DNA source tracking marker for the detection of fecal contamination in harvested rainwater. Sci Total Environ. 2017; 615:99-106. DOI: 10.1016/j.scitotenv.2017.09.229. View

13.

Garner E, McLain J, Bowers J, Engelthaler D, Edwards M, Pruden A . Microbial Ecology and Water Chemistry Impact Regrowth of Opportunistic Pathogens in Full-Scale Reclaimed Water Distribution Systems. Environ Sci Technol. 2018; 52(16):9056-9068. DOI: 10.1021/acs.est.8b02818. View

14.

Bradshaw J, Snyder B, Oladeinde A, Spidle D, Berrang M, Meinersmann R . Characterizing relationships among fecal indicator bacteria, microbial source tracking markers, and associated waterborne pathogen occurrence in stream water and sediments in a mixed land use watershed. Water Res. 2016; 101:498-509. DOI: 10.1016/j.watres.2016.05.014. View

15.

Li X, Harwood V, Nayak B, Staley C, Sadowsky M, Weidhaas J . A novel microbial source tracking microarray for pathogen detection and fecal source identification in environmental systems. Environ Sci Technol. 2015; 49(12):7319-29. DOI: 10.1021/acs.est.5b00980. View

16.

Bernhard A, Field K . Identification of nonpoint sources of fecal pollution in coastal waters by using host-specific 16S ribosomal DNA genetic markers from fecal anaerobes. Appl Environ Microbiol. 2000; 66(4):1587-94. PMC: 92027. DOI: 10.1128/AEM.66.4.1587-1594.2000. View

17.

McClung R, Roth D, Vigar M, Roberts V, Kahler A, Cooley L . Waterborne disease outbreaks associated with environmental and undetermined exposures to water - United States, 2013-2014. Am J Transplant. 2017; 18(1):262-267. DOI: 10.1111/ajt.14607. View

18.

Newton R, Vandewalle J, Borchardt M, Gorelick M, McLellan S . Lachnospiraceae and Bacteroidales alternative fecal indicators reveal chronic human sewage contamination in an urban harbor. Appl Environ Microbiol. 2011; 77(19):6972-81. PMC: 3187108. DOI: 10.1128/AEM.05480-11. View

19.

Donado-Godoy P, Byrne B, Hume M, Leon M, Perez-Gutierrez E, Vives Flores M . Molecular characterization of Salmonella paratyphi B dT+ and Salmonella Heidelberg from poultry and retail chicken meat in Colombia by pulsed-field gel electrophoresis. J Food Prot. 2015; 78(4):802-7. DOI: 10.4315/0362-028X.JFP-14-356. View

20.

Feng S, Bootsma M, McLellan S . Human-Associated Lachnospiraceae Genetic Markers Improve Detection of Fecal Pollution Sources in Urban Waters. Appl Environ Microbiol. 2018; 84(14). PMC: 6029095. DOI: 10.1128/AEM.00309-18. View