Mutations in the H7 HA and PB1 Genes of Avian Influenza a Viruses Increase Viral Pathogenicity and Contact Transmission in Guinea Pigs

Overview

Journal Emerg Microbes Infect

Specialty Infectious Diseases

Date 2019 Sep 11

PMID 31503518

Citations 7

Authors

Carola Dreier

Patricia Resa-Infante

Swantje Thiele

Stephanie Stanelle-Bertram

Kerstin Walendy-Gnirss

Thomas Speiseder

Annette Preuss

Zacharias Muller

Hans-Dieter Klenk

Jurgen Stech

Gulsah Gabriel

Affiliations

Soon will be listed here.

Abstract

Avian influenza A viruses (AIV) of the H7 subtype continue to evolve posing a pandemic threat. However, molecular markers of H7N7 AIV pathogenicity and transmission in mammals remain poorly understood. In this study, we performed a systematic and analysis by comparing an H7N7 highly pathogenic AIV and its ferret adapted variant. Passaging an H7N7 AIV in ferrets led to six mutations in genes encoding the viral polymerase complex and the viral surface proteins. Here, we show that mutations in the H7 hemagglutinin gene cause increased pathogenicity in mice. Contact transmission between guinea pigs required additional mutations in the gene encoding the polymerase subunit PB1. Thus, particular vigilance is required with respect to HA and PB1 mutations as predictive molecular markers to assess the pandemic risk posed by emerging H7 avian influenza viruses.

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