Impact of Extract and Magnetized Water on the Survival Rate and Functional Capabilities of Pancreatic β-cells in Type 2 Diabetic Rat Model

Overview

Journal Diabetes Metab Syndr Obes

Publisher Dove Medical Press

Specialty Endocrinology

Date 2019 Sep 10

PMID 31496771

Citations 5

Authors

Ahmed Saleh

Mamdouh M Anwar

Ahmed E Zayed

Manal El Sayed Ezz Eldeen

Gamal Afifi

Hassien M Alnashiri

Asmaa Ms Gomaa

Mahmoud Abd-Elkareem

Alaa Sayed Abou-Elhamd

Emad S Shaheen

Ghada A Mohamed

Helal F Hetta

Ahmed M Kotb

Affiliations

Soon will be listed here.

Abstract

Introduction: Type 2 diabetes (T2D) is a widely distributed disease that affects large population worldwide. This study aimed to verify the role of (GB) extract and magnetized water (MW) on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rats.

Materials And Methods: T2D was induced by feeding the rats on a high-fat diet (20% fat, 45% carbohydrate, 22% protein) for eight weeks followed by intra-peritoneal injection of a single low dose of streptozotocin (25mg/Kg). Forty rats were randomly assigned to four groups (n=10 rats) as follows: non treated control and three diabetic groups. One diabetic group served as a positive control (diabetic), while the other two groups were orally administered with water extract of GB leaves (0.11 g/kg/day) and MW (600 gauss) for four weeks, respectively.

Results: The β-cell mass and insulin expression in these cells increased markedly after both treatments, particularly in GB treated group. In addition, the immune-expression of the two antioxidant enzymes; glutathione and superoxide dismutase 2 (SOD2) in the pancreatic tissue demonstrated a down-regulation in GB and MW treated groups as compared with the diabetic group.

Conclusion: A four-week treatment of GB and MW protected pancreatic β-cell cells and improved their insulin expression and antioxidant status in type 2 diabetic rats.

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