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Binding of Swine IgM Immunoglobulins to Peripheral Nerve Myelin Sheaths in Electron Microscopic Immunocytochemistry

Overview
Journal Acta Neuropathol
Specialty Neurology
Date 1988 Jan 1
PMID 3149120
Citations 2
Authors
C Sommer
J M Schroder
Affiliations
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Abstract

The IgM fraction of normal swine serum (NSS) was found to adhere to human peripheral nerve myelin sheaths from randomly selected neuropathies and control nerves in electron microscopic immunocytochemical assays. The reaction could be blocked by preincubation with undiluted fetal calf serum (FCS), whereas endoneurial background staining was already abolished by preincubation with 10% FCS. Only the IgM, but not the IgG or the IgA fraction of NSS adhered to peripheral nerve tissue. Such strong adherence of one of the components in an immunocytochemical assay may be a source of misinterpretation. Binding of swine IgM to human peripheral nerve myelin has not been described before and is of interest in the discussion about IgM binding to myelin-associated glycoprotein in IgM gammopathies.

Citing Articles

Immunoelectron microscopic localization of monoclonal IgM antibodies in gammopathy associated with peripheral demyelinative neuropathy.

Lach B, Rippstein P, ATACK D, Afar D, Gregor A Acta Neuropathol. 1993; 85(3):298-307.

PMID: 8384775 DOI: 10.1007/BF00227726.

Polyneuropathy associated with IgM monoclonal gammopathy. Immunological and pathological study in 31 patients.

Vital A, Vital C, Julien J, Baquey A, Steck A Acta Neuropathol. 1989; 79(2):160-7.

PMID: 2480693 DOI: 10.1007/BF00294374.

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