An Improved Rapid Sampling Microdialysis System for Human and Porcine Organ Monitoring in a Hospital Setting

Overview

Journal Anal Methods

Specialties Biochemistry
Chemistry

Date 2019 Sep 7

PMID 31490460

Citations 6

Authors

Sally A N Gowers

Karim Hamaoui

Natalie Vallant

George B Hanna

Ara Darzi

Daniel Casanova

Vassilios Papalois

Martyn G Boutelle

Affiliations

Soon will be listed here.

Abstract

Online organ monitoring could provide clinicians with critical information regarding organ health prior to transplantation and could aid clinical decision-making. This paper presents the methodology of online microdialysis for real-time monitoring of human organs . We describe how rapid sampling microdialysis can be incorporated with organ perfusion machines to create a robust organ monitoring system and demonstrate its use in monitoring human and porcine kidneys as well as human and porcine pancreases. In this paper we also show the potential usefulness of this methodology for evaluating novel interventions in a research setting. The analysis system can be configured either to analyse two analytes in one organ, allowing for ratiometric analysis, or alternatively to monitor one analyte in two organs simultaneously, allowing direct comparison. It was found to be reliable over long monitoring periods in real clinical use. The results clearly show that the analysis system is sensitive to differences between organs and therefore has huge potential as an organ monitoring tool.

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References

Rogers M, Brennan P, Leong C, Gowers S, Aldridge T, Mellor T . Online rapid sampling microdialysis (rsMD) using enzyme-based electroanalysis for dynamic detection of ischaemia during free flap reconstructive surgery. Anal Bioanal Chem. 2013; 405(11):3881-8. PMC: 3608874. DOI: 10.1007/s00216-013-6770-z. View

Ojo A, Hanson J, Meier-Kriesche H, Okechukwu C, Wolfe R, Leichtman A . Survival in recipients of marginal cadaveric donor kidneys compared with other recipients and wait-listed transplant candidates. J Am Soc Nephrol. 2001; 12(3):589-597. DOI: 10.1681/ASN.V123589. View

Rogers M, Leong C, Gowers S, Samper I, Jewell S, Khan A . Simultaneous monitoring of potassium, glucose and lactate during spreading depolarization in the injured human brain - Proof of principle of a novel real-time neurochemical analysis system, continuous online microdialysis. J Cereb Blood Flow Metab. 2016; 37(5):1883-1895. PMC: 5414898. DOI: 10.1177/0271678X16674486. View

Boutelle M, Fellows L, Cook C . Enzyme packed bed system for the on-line measurement of glucose, glutamate, and lactate in brain microdialysate. Anal Chem. 1992; 64(17):1790-4. DOI: 10.1021/ac00041a010. View

Nandi P, Lunte S . Recent trends in microdialysis sampling integrated with conventional and microanalytical systems for monitoring biological events: a review. Anal Chim Acta. 2009; 651(1):1-14. PMC: 3744124. DOI: 10.1016/j.aca.2009.07.064. View

Vander Weele C, Porter-Stransky K, Mabrouk O, Lovic V, Singer B, Kennedy R . Rapid dopamine transmission within the nucleus accumbens: dramatic difference between morphine and oxycodone delivery. Eur J Neurosci. 2014; 40(7):3041-3054. PMC: 4358739. DOI: 10.1111/ejn.12709. View

Schultz K, Kennedy R . Time-resolved microdialysis for in vivo neurochemical measurements and other applications. Annu Rev Anal Chem (Palo Alto Calif). 2010; 1:627-61. DOI: 10.1146/annurev.anchem.1.031207.113047. View

Lee C, Mangino M . Preservation methods for kidney and liver. Organogenesis. 2010; 5(3):105-12. PMC: 2781089. DOI: 10.4161/org.5.3.9582. View

Feuerstein D, Manning A, Hashemi P, Bhatia R, Fabricius M, Tolias C . Dynamic metabolic response to multiple spreading depolarizations in patients with acute brain injury: an online microdialysis study. J Cereb Blood Flow Metab. 2010; 30(7):1343-55. PMC: 2949215. DOI: 10.1038/jcbfm.2010.17. View

10.

Deeba S, Corcoles E, Hanna G, Hanna B, Pareskevas P, Aziz O . Use of rapid sampling microdialysis for intraoperative monitoring of bowel ischemia. Dis Colon Rectum. 2008; 51(9):1408-13. DOI: 10.1007/s10350-008-9375-4. View

11.

Brook N, Nicholson M . Kidney transplantation from non heart-beating donors. Surgeon. 2004; 1(6):311-22. DOI: 10.1016/s1479-666x(03)80065-3. View

12.

Watson C, Venton B, Kennedy R . In vivo measurements of neurotransmitters by microdialysis sampling. Anal Chem. 2006; 78(5):1391-9. DOI: 10.1021/ac0693722. View

13.

Feuerstein D, Parker K, Boutelle M . Practical methods for noise removal: applications to spikes, nonstationary quasi-periodic noise, and baseline drift. Anal Chem. 2009; 81(12):4987-94. DOI: 10.1021/ac900161x. View

14.

Scott D, Grigsby R, Lunte S . Microdialysis sampling coupled to microchip electrophoresis with integrated amperometric detection on an all-glass substrate. Chemphyschem. 2013; 14(10):2288-94. PMC: 4000424. DOI: 10.1002/cphc.201300449. View

15.

Smit M, Cass A . Cyanide detection using a substrate-regenerating, peroxidase-based biosensor. Anal Chem. 1990; 62(22):2429-36. DOI: 10.1021/ac00221a004. View

16.

Hamaoui K, Gowers S, Damji S, Rogers M, Leong C, Hanna G . Rapid sampling microdialysis as a novel tool for parenchyma assessment during static cold storage and hypothermic machine perfusion in a translational ex vivo porcine kidney model. J Surg Res. 2015; 200(1):332-45. DOI: 10.1016/j.jss.2015.07.004. View

17.

Hamaoui K, Gowers S, Boutelle M, Cook T, Hanna G, Darzi A . Organ Pretreatment With Cytotopic Endothelial Localizing Peptides to Ameliorate Microvascular Thrombosis and Perfusion Deficits in Ex Vivo Renal Hemoreperfusion Models. Transplantation. 2016; 100(12):e128-e139. DOI: 10.1097/TP.0000000000001437. View

18.

St Peter S, Imber C, Friend P . Liver and kidney preservation by perfusion. Lancet. 2002; 359(9306):604-13. DOI: 10.1016/S0140-6736(02)07749-8. View

19.

Taylor M, Baicu S . Current state of hypothermic machine perfusion preservation of organs: The clinical perspective. Cryobiology. 2009; 60(3 Suppl):S20-35. PMC: 2891866. DOI: 10.1016/j.cryobiol.2009.10.006. View

20.

Port F, Bragg-Gresham J, Metzger R, Dykstra D, Gillespie B, Young E . Donor characteristics associated with reduced graft survival: an approach to expanding the pool of kidney donors. Transplantation. 2002; 74(9):1281-6. DOI: 10.1097/00007890-200211150-00014. View