Myosin II Isoforms Play Distinct Roles in Junction Biogenesis

Overview

Journal Elife

Specialty Biology

Date 2019 Sep 6

PMID 31486768

Citations 38

Authors

Melina L Heuze

Gautham Hari Narayana Sankara Narayana

Joseph dAlessandro

Victor Cellerin

Tien Dang

David S Williams

Jan Cm Van Hest

Philippe Marcq

Rene-Marc Mege

Benoit Ladoux

Affiliations

Soon will be listed here.

Abstract

Adherens junction (AJ) assembly under force is essential for many biological processes like epithelial monolayer bending, collective cell migration, cell extrusion and wound healing. The acto-myosin cytoskeleton acts as a major force-generator during the de novo formation and remodeling of AJ. Here, we investigated the role of non-muscle myosin II isoforms (NMIIA and NMIIB) in epithelial junction assembly. NMIIA and NMIIB differentially regulate biogenesis of AJ through association with distinct actin networks. Analysis of junction dynamics, actin organization, and mechanical forces of control and knockdown cells for myosins revealed that NMIIA provides the mechanical tugging force necessary for cell-cell junction reinforcement and maintenance. NMIIB is involved in E-cadherin clustering, maintenance of a branched actin layer connecting E-cadherin complexes and perijunctional actin fibres leading to the building-up of anisotropic stress. These data reveal unanticipated complementary functions of NMIIA and NMIIB in the biogenesis and integrity of AJ.

Citing Articles

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