Human Intestinal Enteroids Model MHC-II in the Gut Epithelium

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2019 Sep 5

PMID 31481960

Citations 18

Authors

Jonathan E Wosen

Alexandra Ilstad-Minnihan

Julia Y Co

Wei Jiang

Dhriti Mukhopadhyay

Nielsen Q Fernandez-Becker

Calvin J Kuo

Manuel R Amieva

Elizabeth D Mellins

Affiliations

Soon will be listed here.

Abstract

The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, "mini-guts" derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel studies into IEC MHC-II regulation and function during health and disease.

Citing Articles

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